Goyns M H
Biochem J. 1979 Dec 15;184(3):607-12. doi: 10.1042/bj1840607.
The polyamine content of the circulating erythrocyte population in the embryonic chick was studied during its development. Total cellular polyamine content fell dramatically between 5 and 7 days of development, paralleling the decrease in metabolic activity exhibited by these cells. Nuclei were isolated from the erythrocytes by a non-aqueous technique, which not only eliminated the polyamine loss that occurred with aqueous isolation, but also prevented redistribution of the polyamines from the cytoplasm. Nuclear spermidine and spermine contents decreased markedly between 5 and 6 days of development from 31 to 10 pmol/microgram of DNA and from 33 to 18 pmol/microgram of DNA respectively. Thereafter the spermine content remained constant, but the spermidine content continued to decline. Good correlations between spermidine and RNA contents were observed in both cells and nuclei, and similarly between spermine and RNA contents in cells, but no such correlation was observed between spermine and RNA in nuclei.
在胚胎期雏鸡发育过程中，对其循环红细胞群体中的多胺含量进行了研究。在发育的第5至7天，细胞内多胺总量急剧下降，这与这些细胞代谢活性的降低相一致。采用非水技术从红细胞中分离细胞核，该技术不仅消除了水相分离时发生的多胺损失，还防止了多胺从细胞质中重新分布。在发育的第5至6天，细胞核中的亚精胺和精胺含量显著下降，分别从31皮摩尔/微克DNA降至10皮摩尔/微克DNA，从33皮摩尔/微克DNA降至18皮摩尔/微克DNA。此后，精胺含量保持恒定，但亚精胺含量继续下降。在细胞和细胞核中均观察到亚精胺与RNA含量之间有良好的相关性，在细胞中精胺与RNA含量之间也有类似的相关性，但在细胞核中精胺与RNA之间未观察到这种相关性。
