Tsukada Y, Hirai H
Ann N Y Acad Sci. 1975 Aug 22;259:37-44. doi: 10.1111/j.1749-6632.1975.tb25400.x.
The synthesis of alpha-fetoprotein and albumin in two clones of AH66 hepatoma was studied. (1) Amounts of AFP and albumin synthesized by the C-4 clone were 2.9 and 0.28 X 10(-7) mug per cell per hour, respectively. AFP and albumin amounts synthesized by the A-1 clone were 2.5 and 1.8 X 10(-7) mug per cell per hour, respectively. (2) The cell cycles of the C-4 and A-1 clones were as follows: C-4 clone: mean generation time 20.5 hours: G1, 10 hours; S, 7 hours; G2, 4 hours; and M, 30 minutes. A-1 clone: mean generation time 50.7 hours: G1, 36 hours; S, 10 hours; G2, 4 hours; and M, 30-60 minutes. (3) AFP was found to be synthesized from late G1 phase to the end of the S phase for 9 hours in C-4 and 25 hours in A-1. The albumin production was from late S phase to the beginning of the G2 phase for 4 hours in C-4 and 9 hours in A-1, which were approximately half or one-third of the time spent on AFP production. (4) The double staining with fluorescent-conjugated antibodies to AFP and albumin demonstrated that AFP and albumin are probably synthesized by different cells.
对AH66肝癌的两个克隆株中甲胎蛋白和白蛋白的合成进行了研究。（1）C - 4克隆株每细胞每小时合成的甲胎蛋白和白蛋白量分别为2.9和0.28×10⁻⁷微克。A - 1克隆株每细胞每小时合成的甲胎蛋白和白蛋白量分别为2.5和1.8×10⁻⁷微克。（2）C - 4和A - 1克隆株的细胞周期如下：C - 4克隆株：平均世代时间20.5小时，G1期10小时，S期7小时，G2期4小时，M期30分钟。A - 1克隆株：平均世代时间50.7小时，G1期36小时，S期10小时，G2期4小时，M期30 - 60分钟。（3）发现C - 4克隆株中甲胎蛋白从G1期晚期到S期末合成9小时，A - 1克隆株中为25小时。白蛋白合成在C - 4克隆株中从S期末到G2期开始持续4小时，在A - 1克隆株中为9小时，约为甲胎蛋白合成时间的一半或三分之一。（4）用荧光偶联的甲胎蛋白和白蛋白抗体进行双重染色表明，甲胎蛋白和白蛋白可能由不同细胞合成。
