Adair F W
J Bacteriol. 1966 Oct;92(4):899-904. doi: 10.1128/jb.92.4.899-904.1966.
Adair, Frank W. (Rutgers, The State University, New Brunswick, N. J.). Membrane-associated sulfur oxidation by the autotroph Thiobacillus thiooxidans. J. Bacteriol. 92:899-904. 1966.-Washed cell wall-membrane fragments derived from sulfur-grown cells of the strictly autotrophic bacterium, Thiobacillus thiooxidans, oxidized elemental sulfur to sulfate without the addition of cofactors. The oxidation was optimal at pH 7.0 and was increased by the presence of wetting agents. Oxygen uptake was inhibited by cyanide, azide, and thiol-binding agents. Sulfite was also oxidized, and both the sulfur- and sulfite-oxidizing systems were heat-labile. Neither thiosulfate nor tetrathionate was oxidized by soluble or membrane preparations. The fragments fixed C(14)O(2) in the presence of ribose-5-phosphate, Mg(++), and adenosine triphosphate. Sulfur oxidation did not provide energy for C(14)O(2) fixation in this system.
阿代尔,弗兰克·W.(罗格斯大学,新泽西州立大学,新不伦瑞克)。自养型氧化硫硫杆菌的膜相关硫氧化作用。《细菌学杂志》92:899 - 904。1966年。——从严格自养型细菌氧化硫硫杆菌的硫生长细胞中提取的洗涤过的细胞壁 - 膜片段,在不添加辅因子的情况下将元素硫氧化为硫酸盐。氧化作用在pH 7.0时最佳,并且在有湿润剂存在时会增强。氰化物、叠氮化物和硫醇结合剂会抑制氧气摄取。亚硫酸盐也会被氧化,并且硫氧化和亚硫酸盐氧化系统都对热不稳定。硫代硫酸盐和连四硫酸盐都不会被可溶性制剂或膜制剂氧化。这些片段在核糖 - 5 - 磷酸、Mg(++)和三磷酸腺苷存在的情况下固定C(14)O(2)。在该系统中,硫氧化不为C(14)O(2)固定提供能量。