Larsen H J
Res Vet Sci. 1979 Nov;27(3):334-8.
A simple and reproducible micromethod for determination of in vitro mitogenic responses of sheep peripheral blood lymphocytes is described. The test uses (i) whole blood diluted in RPMI 1640 medium to give a cell count of 0.5 x 106-1 x 106 lymphocytes/ml, (ii) mitogens in the range of 5-20 micrograms of phytohaemagglutinin/ml, 20-80 micrograms of lipopolysaccharide/ml or 20-80 microliters of poke weed mitogen/ml, and (iii) a stimulation time of 42 to 90 h. A considerable variation in mitogenic response was observed both between animals and on different occasions in the same animal. Because of the large periodic variation it was suggested that the test should be repeated using blood drawn at different times in order to determine the mitogenic response of an animal.
本文描述了一种简单且可重复的微量方法，用于测定绵羊外周血淋巴细胞的体外促有丝分裂反应。该试验采用：（i）用RPMI 1640培养基稀释全血，使淋巴细胞计数达到0.5×10⁶ - 1×10⁶个/ml；（ii）丝裂原，其浓度范围为5 - 20微克植物血凝素/ml、20 - 80微克脂多糖/ml或20 - 80微升商陆丝裂原/ml；（iii）刺激时间为42至90小时。在不同动物之间以及同一动物在不同时间均观察到促有丝分裂反应存在相当大的差异。由于存在较大的周期性变化，建议在不同时间采集血液重复该试验，以确定动物的促有丝分裂反应。
