Crowshaw K, Szlyk J Z
Biochem J. 1970 Feb;116(3):421-4. doi: 10.1042/bj1160421.
Three prostaglandins (PGE(2), PGF(2alpha) and PGA(2)) are present in rabbit kidney medulla. An acidic lipid extract (0.165g) obtained from 2kg of frozen rabbit kidney cortex was separated by silicic acid chromatography to yield eluates containing fatty acids, possible non-polar prostaglandin metabolites, PGA, PGE and PGF compounds. Ultraviolet spectra of the eluates before and after treatment with sodium hydroxide did not yield chromophores typical of any known prostaglandins or related metabolites. By using more sensitive bioassay procedures (contraction of rabbit duodenum) weak activity equivalent to 60mug of PGE(2) and 10mug of PGF(2alpha) was detected in the PGE and PGF eluates respectively. Extraction and bioassay of fresh kidney cortex revealed no prostaglandin-like activity. Attempts to biosynthesize prostaglandins in fresh homogenates of rabbit kidney cortex from endogenous precursors and from added arachidonic acid were unsuccessful. When freshly prepared homogenates of rabbit kidney cortex were incubated with added PGE(1) no evidence of enzymic breakdown was obtained. It is concluded that rabbit kidney prostaglandins are present predominantly in the medulla and there are no cortical mechanisms for their biosynthesis or inactivation under normal conditions.
三种前列腺素（前列腺素E₂、前列腺素F₂α和前列腺素A₂）存在于兔肾髓质中。从2千克冷冻兔肾皮质中获得的酸性脂质提取物（0.165克）通过硅酸色谱法进行分离，得到含有脂肪酸、可能的非极性前列腺素代谢物、前列腺素A、前列腺素E和前列腺素F化合物的洗脱液。用氢氧化钠处理前后洗脱液的紫外光谱未产生任何已知前列腺素或相关代谢物的典型发色团。通过使用更灵敏的生物测定方法（兔十二指肠收缩），在前列腺素E和前列腺素F洗脱液中分别检测到相当于60微克前列腺素E₂和10微克前列腺素F₂α的微弱活性。新鲜肾皮质的提取和生物测定未显示出前列腺素样活性。尝试从内源性前体和添加的花生四烯酸在兔肾皮质新鲜匀浆中生物合成前列腺素未成功。当将新鲜制备的兔肾皮质匀浆与添加的前列腺素E₁一起孵育时，未获得酶促分解的证据。得出的结论是，兔肾前列腺素主要存在于髓质中，在正常条件下皮质中不存在其生物合成或失活机制。
