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用于超离心机防护的III级生物安全柜评估

Evaluation of a class III biological safety cabinet for enclosure of an ultracentrifuge.

作者信息

Chatigny M A, Dunn S, Ishimaru K, Eagleson J A, Prusiner S B

出版信息

Appl Environ Microbiol. 1979 Nov;38(5):934-9. doi: 10.1128/aem.38.5.934-939.1979.

DOI:10.1128/aem.38.5.934-939.1979
PMID:543704
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC243612/
Abstract

An evaluation of a special safety cabinet housing a high-speed centrifuge was made. The cabinet enclosed both the top access port and the drive and pumping machinery of the centrifuge. A titanium rotor was loaded with tubes containing a bacterial culture, weakened, and driven until rotor rupture occurred. There were several bent and broken components in the centrifuge, and bacteria leaked from the vacuum chamber. Although the forces were sufficient to displace the cabinet, none of the test bacteria were found outside the cabinet.

摘要

对一个装有高速离心机的特殊安全柜进行了评估。该柜子封闭了离心机的顶部入口以及驱动和泵送机械装置。一个钛制转子装载着装有细菌培养物的试管,转子被加速直至破裂。离心机中有几个部件弯曲和损坏,细菌从真空室泄漏出来。尽管产生的力量足以移动柜子,但在柜子外部未发现任何测试细菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d773/243612/cab91293a6b6/aem00204-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d773/243612/78e936fb9e0b/aem00204-0180-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d773/243612/f59033ec66b7/aem00204-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d773/243612/cab91293a6b6/aem00204-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d773/243612/78e936fb9e0b/aem00204-0180-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d773/243612/f59033ec66b7/aem00204-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d773/243612/cab91293a6b6/aem00204-0182-a.jpg

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本文引用的文献

1
Aerosol production associated with clinical laboratory procedures.
Am J Clin Pathol. 1974 Nov;62(5):591-600. doi: 10.1093/ajcp/62.5.591.