微生物安全柜中微生物气溶胶的控制
Containment of microbial aerosols in a microbiological safety cabinet.
作者信息
Barbeito M S, Taylor L A
出版信息
Appl Microbiol. 1968 Aug;16(8):1225-9. doi: 10.1128/am.16.8.1225-1229.1968.
A microbiological safety cabinet was evaluated to determine conditions under which microorganisms might escape. Tests were conducted under three cabinet-closure conditions, various airflow velocities, and different laboratory operations, with 10(5), 1.1 x 10(5), and 10(6) microorganisms per cubic foot of cabinet space released per min for 5 min. The data revealed that (i) escape of a human infectious dose is possible when the cabinet is used with the glove panel off; (ii) the number of organisms that escaped from the cabinet increased with a decrease in air velocity; and (iii) an increase in the number of laboratory operations resulted in an increase in the number of organisms that escaped. Thus, when the glove panel was off, the cabinet was only safe for operations that released a small number of microorganisms into the cabinet, whereas the cabinet was safe for operations of significantly greater hazard when used with the glove panel on but with the gloves unattached.
对一台微生物安全柜进行了评估，以确定微生物可能逸出的条件。在三种柜体关闭条件、不同气流速度和不同实验室操作情况下进行了测试，每分钟每立方英尺柜体空间释放10⁵、1.1×10⁵和10⁶个微生物，持续5分钟。数据显示：（i）当安全柜在手套板打开的情况下使用时，有可能逸出具有人类感染剂量的微生物；（ii）从安全柜逸出的微生物数量随着空气流速的降低而增加；（iii）实验室操作数量的增加导致逸出的微生物数量增加。因此，当手套板打开时，该安全柜仅对向柜内释放少量微生物的操作是安全的，而当手套板关闭但不戴手套使用时，该安全柜对危险性显著更高的操作是安全的。
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