Macher J M, First M W
Appl Environ Microbiol. 1984 Sep;48(3):481-5. doi: 10.1128/aem.48.3.481-485.1984.
Biological safety cabinets are frequently relied upon to provide sterile work environments in which hazardous microorganisms can be safely handled. Verification of correct airstream velocities does not, by itself, ensure that adequate protection will be achieved under all users. Instead, the concentration of microorganisms in a cabinet operator's breathing zone must be measured during typical cabinet use conditions to determine whether the exposure is below acceptable limits. In this study, cabinet operator exposures were measured with a personal air sampler. Bacterial spores were released inside a cabinet as a uniform challenge aerosol, and the number of escaping spores was measured for several cabinet arrangements during a number of typical operations. The following were studied to determine their effects on aerosol containment: inflow air velocity, size of access opening, type of operator movements, location of operator's hands, and pace of activity. Other experiments examined differences in aerosol containment for eight typical microbiology operations when performed by six operators who covered a range of body heights and volumes.
生物安全柜常用于提供无菌的工作环境，以便能安全地处理有害微生物。仅验证气流速度正确，并不能确保在所有用户使用情况下都能提供充分的防护。相反，必须在生物安全柜的典型使用条件下，测量柜内操作人员呼吸区域内的微生物浓度，以确定暴露水平是否低于可接受限度。在本研究中，使用个人空气采样器测量柜内操作人员的暴露情况。在生物安全柜内释放细菌孢子，形成均匀的挑战气溶胶，并在若干典型操作过程中，针对多种柜内布局测量逸出孢子的数量。研究了以下因素对气溶胶 containment 的影响：进气流速、入口开口大小、操作人员动作类型、操作人员手部位置以及活动节奏。其他实验还考察了由六名身高和体型各异的操作人员进行八项典型微生物学操作时，气溶胶 containment 的差异。
