福尔马林、β-丙内酯、硫柳汞和紫外线对流感病毒感染性、鸡细胞凝集、血凝及抗原性的影响。
Effect of formalin, beta-propiolactone, merthiolate, and ultraviolet light upon influenza virus infectivity chicken cell agglutination, hemagglutination, and antigenicity.
作者信息
Goldstein M A, Tauraso N M
出版信息
Appl Microbiol. 1970 Feb;19(2):290-4. doi: 10.1128/am.19.2.290-294.1970.
Abstract
Four strains of influenza virus were treated with Formalin, Merthiolate, Merthiolate and Formalin, ultraviolet light, and beta-propiolactone (BPL) for 18, 48, and 72 hr. Infectivity, chicken cell agglutination (CCA), hemagglutination (HA), and antigenicity determinations were made. Except for Merthiolate, each method of inactivation was equally effective in reducing infectivity. Loss of infectivity was related to length of treatment. CCA determinations were higher for all treated groups except for BPL-treated samples; these had lower determinations. BPL treatment also lowered the HA titer. Antigenicity was lessened by BPL treatment and by Merthiolate and Formalin treatment. Generally, the length of inactivation up to 72 hr did not affect CCA, HA, or antigenicity determinations. For the most part, there was no significant differences in the reactivity of the four strains.
摘要
四株流感病毒分别用福尔马林、硫柳汞、硫柳汞与福尔马林、紫外线和β-丙内酯（BPL）处理18、48和72小时。进行了感染性、鸡细胞凝集（CCA）、血凝（HA）和抗原性测定。除硫柳汞外，每种灭活方法在降低感染性方面同样有效。感染性的丧失与处理时间有关。除BPL处理的样本外，所有处理组的CCA测定值都较高；这些样本的测定值较低。BPL处理也降低了HA滴度。BPL处理以及硫柳汞和福尔马林处理都会降低抗原性。一般来说，长达72小时的灭活时间不会影响CCA、HA或抗原性测定。在大多数情况下，这四株病毒的反应性没有显著差异。
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Effect of formalin, beta-propiolactone, merthiolate, and ultraviolet light upon influenza virus infectivity chicken cell agglutination, hemagglutination, and antigenicity.福尔马林、β-丙内酯、硫柳汞和紫外线对流感病毒感染性、鸡细胞凝集、血凝及抗原性的影响。
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