Svennevig J L, Svaar H
Int J Cancer. 1979 Dec 15;24(6):754-8. doi: 10.1002/ijc.2910240609.
The relative content of lymphocytes and macrophages was studied in 20 solid malignant human tumours. Mechanically prepared tumour-cell suspensions contained both lymphocytes (0.2-4.0%) and macrophages (0.2-7.0%). Macrophages were characterized as esterase-positive, phagocytosing cells. The distribution of the cells within the tumours was studied on cryostat sections. A modified esterase method, using alpha-naphthyl butyrate as substrate, always gave an intensive staining of tissue-infiltrating macrophages, even in malignant tissues where some neoplastic cells exhibited a weak to moderate esterase activity. Tumour-infiltrating lymphocytes were studied in HE-stained tissue sections. The study demonstrates that both cell types were usually more numerous in the stroma surrounding the tumour tissue and in the stromal septa between the cords of malignant cells (peripheral infiltration) than in the central areas of the tumours. No signs of cell necrosis were seen near the lymphocyte zones or the stromal macrophages; however, some central necrotic areas contained aggregates of macrophages, lymphocytes and PMN.
对20例人类实体恶性肿瘤中的淋巴细胞和巨噬细胞的相对含量进行了研究。机械制备的肿瘤细胞悬液中同时含有淋巴细胞（0.2 - 4.0%）和巨噬细胞（0.2 - 7.0%）。巨噬细胞的特征为酯酶阳性且具有吞噬作用的细胞。在冷冻切片上研究了肿瘤内细胞的分布情况。一种改良的酯酶方法，以α - 萘丁酸作为底物，即使在一些肿瘤细胞表现出弱至中度酯酶活性的恶性组织中，也总能使浸润组织的巨噬细胞得到强烈染色。在苏木精 - 伊红（HE）染色的组织切片中研究肿瘤浸润淋巴细胞。该研究表明，这两种细胞类型通常在肿瘤组织周围的间质以及恶性细胞索之间的间质隔（外周浸润）中比在肿瘤的中心区域更为丰富。在淋巴细胞区域或间质巨噬细胞附近未见细胞坏死迹象；然而，一些中央坏死区域含有巨噬细胞、淋巴细胞和多形核中性粒细胞（PMN）的聚集物。
