Müller J, Keller H U, Dürig P, Hagmann J, Cornioley D M, Reinhard J, Ruchti C, Hess M W, Cottier H
Int Arch Allergy Appl Immunol. 1981;64(4):410-21. doi: 10.1159/000232721.
A substantial proportion of human peripheral lymphocytes exhibits esterase activity as demonstrated by cytochemical techniques using hexazotized pararosanilin as coupling agent and different alpha-naphthyl compounds, in particular alpha-naphthyl-acetate, as substrates. Esterase-positive lymphocytes showed one or several dots of the reaction product. Plasma cells and macrophages also exhibited marked esterase activity but with diffuse distribution of the reaction product throughout the cytoplasm. The substrate specificity, the time course and the pH optimum of the cytochemical reaction were determined. The results indicate that these lymphocyte esterases are identical with acid lipases. The number of esterase-positive cells reaches a plateau well below 100% (85% for blood lymphocytes) even with optimal staining procedures. The relation between esterase activity and other so-called lymphocyte markers such as sIg and the capacity to form spontaneous rosettes with sheep red blood cells was investigated in peripheral human blood lymphocytes. A fair correlation between rosette formation and esterase activity was found and most of the esterase-positive cells were sIg-negative. However, the fit was never complete. The results suggest that esterase activity is largely characteristic for small peripheral T cells. Almost all blast cells formed following stimulation with Con A or PHA were esterase-positive. Histochemical studies showed that the large majority of the thymocytes in the cortex lacked esterase activity. In lymph nodes, a low proportion of esterase-positive cells was found in the germinal centers, whereas most lymphocytes in the paracortical are were esterase-positive.
运用以重氮副蔷薇苯胺作为偶联剂、不同的α-萘基化合物（尤其是α-萘基乙酸）作为底物的细胞化学技术表明，相当比例的人外周淋巴细胞呈现酯酶活性。酯酶阳性淋巴细胞显示出一个或几个反应产物点。浆细胞和巨噬细胞也呈现出显著的酯酶活性，但反应产物在整个细胞质中呈弥漫分布。确定了细胞化学反应的底物特异性、时间进程和最适pH值。结果表明，这些淋巴细胞酯酶与酸性脂肪酶相同。即使采用最佳染色程序，酯酶阳性细胞的数量也远低于100%达到平台期（血液淋巴细胞为85%）。对外周血淋巴细胞中酯酶活性与其他所谓淋巴细胞标志物（如sIg）以及与绵羊红细胞形成自发玫瑰花结的能力之间的关系进行了研究。发现玫瑰花结形成与酯酶活性之间存在一定相关性，且大多数酯酶阳性细胞为sIg阴性。然而，拟合并不完全。结果表明，酯酶活性在很大程度上是外周小T细胞的特征。用Con A或PHA刺激后形成的几乎所有母细胞都是酯酶阳性的。组织化学研究表明，皮质中的大多数胸腺细胞缺乏酯酶活性。在淋巴结中，生发中心发现低比例的酯酶阳性细胞，而副皮质区的大多数淋巴细胞是酯酶阳性的。
