牛肝自溶过程中酸性磷酸酶的分布
Distribution of acid phosphatase during autolysis in bovine liver.
作者信息
Pekkanen T J
出版信息
Acta Vet Scand. 1970;11(2):283-94. doi: 10.1186/BF03547988.
The percentage distribution of acid phosphatase between lysosomes and cytoplasm in bovine liver during autolysis at 37°C was investigated. The share of the cytoplasmic acid phosphatase activity of the total acid phosphatase activity in liver tissue increased during autolysis being before incubation 28–42 % and after 24 hrs.' incubation at 37°C 63–94 %. Microbiological contamination increased the proportion of cytoplasmic acid phosphatase. When bovine liver was incubated at 37°C for 24 hrs., the activity of the total acid phosphatase decreased to about 50 % of the initial activity. During a 16 days' incubation at 4°C the total acid phosphatase activity of bovine liver, however, remained unchanged.
研究了牛肝在37℃自溶过程中酸性磷酸酶在溶酶体和细胞质之间的百分比分布。肝组织中细胞质酸性磷酸酶活性占总酸性磷酸酶活性的比例在自溶过程中增加，孵育前为28%-42%，在37℃孵育24小时后为63%-94%。微生物污染增加了细胞质酸性磷酸酶的比例。当牛肝在37℃孵育24小时时，总酸性磷酸酶活性降至初始活性的约50%。然而，在4℃孵育16天期间，牛肝的总酸性磷酸酶活性保持不变。
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