Palmblad J, Engstedt L
Acta Pathol Microbiol Scand C. 1979 Dec;87(6):357-64.
In most polymorphonuclear (PMN) bactericidal assays each PMN is given appr. 0.5-4 bacteria to kill but it has not been sufficiently shown that this ratio is optimal for the detection of changes in PMN killing functions. One PMN, incubated with increasing amounts of Staph. aureus, can kill between 45 and 90 colony forming units (CFU). Therefore a new assay, giving each PMN a submaximal bacterial amount (i.e. using 2-4 CFU/PMN) concerning the effects on PMNs of thermal inactivation and of levamisole. Exposure to 46 degrees C for 3-5 minutes decreased the killing capacity, easiest descernable with the standard assay. Incubation with levamisole in concentrations ranging between 10(-6) and 10(-7) mol/l increased the PMN killing capacity, and this was most evident with the modified assay. Thus, enhancements of PMN killing functions might be detected better with the present modified PMN bacterial assay, whereas impairments are disclosed more readily with the standard assay.
在大多数多形核白细胞（PMN）杀菌试验中，每个PMN大约要杀灭0.5 - 4个细菌，但尚未充分证明该比例对于检测PMN杀伤功能的变化是最佳的。一个PMN与数量不断增加的金黄色葡萄球菌一起孵育时，能杀灭45至90个菌落形成单位（CFU）。因此，有一种新的试验，就热灭活和左旋咪唑对PMN的影响而言，给每个PMN的细菌量为次最大量（即使用2 - 4 CFU/PMN）。暴露于46摄氏度3 - 5分钟会降低杀伤能力，这在标准试验中最容易辨别。用浓度在10⁻⁶至10⁻⁷摩尔/升之间的左旋咪唑孵育会增加PMN的杀伤能力，这在改良试验中最为明显。因此，用目前改良的PMN细菌试验可能能更好地检测PMN杀伤功能的增强，而用标准试验更容易发现其损伤。
