酿酒酵母中的半乳糖转运。3. 无转糖基酶细胞中半乳糖摄取的特性：反对转运相关磷酸化的证据
Galactose transport in Saccharomyces cerevisiae. 3. Characteristics of galactose uptake in transferaseless cells: evidence against transport-associated phosphorylation.
作者信息
Kuo S C, Cirillo V P
出版信息
J Bacteriol. 1970 Sep;103(3):679-85. doi: 10.1128/jb.103.3.679-685.1970.
Abstract
The characteristics of the inducible galactose transport system in bakers' yeast were studied in uridine diphosphate, galactose-1-phosphate uridylyl-transferaseless cells. Transferaseless cells transport galactose at the same initial rate as wild-type cells and accumulate a mixture of free galactose and galactose-1-phosphate. The addition of (14)C-labeled galactose to cells preloaded with unlabeled galactose and galactose-1-phosphate results in a higher rate of labeling of the free-sugar pool than of the galactose-1-phosphate pool. These results support other evidence that galactose uptake in bakers' yeast is a carrier-mediated, facilitated diffusion and that phosphorylation is an intracellular event after uptake of the free sugar.
摘要
在缺乏尿苷二磷酸半乳糖-1-磷酸尿苷酰转移酶的细胞中，研究了面包酵母中可诱导半乳糖转运系统的特性。缺乏该转移酶的细胞以与野生型细胞相同的初始速率转运半乳糖，并积累游离半乳糖和半乳糖-1-磷酸的混合物。向预先加载了未标记半乳糖和半乳糖-1-磷酸的细胞中添加（14）C标记的半乳糖，结果显示游离糖池的标记速率高于半乳糖-1-磷酸池。这些结果支持了其他证据，即面包酵母中的半乳糖摄取是载体介导的易化扩散，并且磷酸化是游离糖摄取后的细胞内事件。
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本文引用的文献
1
A sensitive assay for galactokinase in Escherichia coli.一种用于检测大肠杆菌中半乳糖激酶的灵敏测定法。
Anal Biochem. 1962 Jun;3:498-513. doi: 10.1016/0003-2697(62)90083-0.
2
THE ROLE OF POLYPHOSPHATES IN THE TRANSPORT MECHANISM OF GLUCOSE IN YEAST CELLS.多聚磷酸盐在酵母细胞葡萄糖转运机制中的作用
J Gen Physiol. 1964 Sep;48(1):43-60. doi: 10.1085/jgp.48.1.43.
3
ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES.酿酒酵母中控制半乳糖利用的基因的酶表达与遗传连锁
Genetics. 1964 May;49(5):837-44. doi: 10.1093/genetics/49.5.837.
4
Mechanism of glucose transport across the yeast cell membrane.葡萄糖跨酵母细胞膜的转运机制。
J Bacteriol. 1962 Sep;84(3):485-91. doi: 10.1128/jb.84.3.485-491.1962.
5
Transport of some mono- and di-saccharides into yeast cells.一些单糖和双糖进入酵母细胞的转运
Biochem J. 1959 Feb;71(2):233-42. doi: 10.1042/bj0710233.
6
The genetic control of galactose utilization in Saccharomyces.酿酒酵母中半乳糖利用的遗传控制。
J Bacteriol. 1954 Dec;68(6):662-70. doi: 10.1128/jb.68.6.662-670.1954.
7
Studies on uridine-diphosphate-glucose.尿苷二磷酸葡萄糖的研究。
Biochem J. 1952 Jun;51(3):426-30. doi: 10.1042/bj0510426.
8
Affinity and capacity of the glucose carrier in different physiological states of a synchronous culture of baker's yeast.面包酵母同步培养物不同生理状态下葡萄糖载体的亲和力和容量
Folia Microbiol (Praha). 1967;12(2):115-20. doi: 10.1007/BF02896871.
9
Regulation of genes controlling synthesis of the galactose pathway enzymes in yeast.酵母中控制半乳糖途径酶合成的基因调控
Genetics. 1966 Sep;54(3):911-6. doi: 10.1093/genetics/54.3.911.
10
The mechanism of transmembrane glucose transport in yeast: evidence for phosphorylation, associated with transport.酵母中跨膜葡萄糖转运的机制：与转运相关的磷酸化证据。
Arch Biochem Biophys. 1969 Mar;130(1):244-52. doi: 10.1016/0003-9861(69)90030-7.
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