酿酒酵母中的半乳糖转运。3. 无转糖基酶细胞中半乳糖摄取的特性：反对转运相关磷酸化的证据
Galactose transport in Saccharomyces cerevisiae. 3. Characteristics of galactose uptake in transferaseless cells: evidence against transport-associated phosphorylation.
作者信息
Kuo S C, Cirillo V P
出版信息
J Bacteriol. 1970 Sep;103(3):679-85. doi: 10.1128/jb.103.3.679-685.1970.
Abstract
The characteristics of the inducible galactose transport system in bakers' yeast were studied in uridine diphosphate, galactose-1-phosphate uridylyl-transferaseless cells. Transferaseless cells transport galactose at the same initial rate as wild-type cells and accumulate a mixture of free galactose and galactose-1-phosphate. The addition of (14)C-labeled galactose to cells preloaded with unlabeled galactose and galactose-1-phosphate results in a higher rate of labeling of the free-sugar pool than of the galactose-1-phosphate pool. These results support other evidence that galactose uptake in bakers' yeast is a carrier-mediated, facilitated diffusion and that phosphorylation is an intracellular event after uptake of the free sugar.
摘要
在缺乏尿苷二磷酸半乳糖-1-磷酸尿苷酰转移酶的细胞中，研究了面包酵母中可诱导半乳糖转运系统的特性。缺乏该转移酶的细胞以与野生型细胞相同的初始速率转运半乳糖，并积累游离半乳糖和半乳糖-1-磷酸的混合物。向预先加载了未标记半乳糖和半乳糖-1-磷酸的细胞中添加（14）C标记的半乳糖，结果显示游离糖池的标记速率高于半乳糖-1-磷酸池。这些结果支持了其他证据，即面包酵母中的半乳糖摄取是载体介导的易化扩散，并且磷酸化是游离糖摄取后的细胞内事件。
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