酿酒酵母半乳糖阴性菌株中溶质渗透性与渗透修复能力之间的关系。
Relationship between solute permeability and osmotic remediability in a galactose-negative strain of Saccharomyces cerevisiae.
作者信息
Bassel J, Douglas H C
出版信息
J Bacteriol. 1970 Nov;104(2):707-11. doi: 10.1128/jb.104.2.707-711.1970.
Abstract
An osmotic remedial allele, gal 7-1, in the galactose pathway of Saccharomyces cerevisiae responds to either penetrating (ethylene glycol and diethylene glycol) or nonpenetrating (KCl, NaCl, and sorbitol) solutes in the growth medium. Extracts from cells grown under restrictive conditions gave no increase in enzyme activity (gal-1-phosphate, uridylyl transferase) when exposed to the penetrating solutes; thus protein synthesis or possibly polymer assembly is proposed as the critical step remedied by the addition of the solutes.
摘要
酿酒酵母半乳糖代谢途径中的一个渗透修复等位基因gal 7-1，对生长培养基中的渗透性溶质（乙二醇和二甘醇）或非渗透性溶质（氯化钾、氯化钠和山梨醇）均有反应。在限制性条件下生长的细胞提取物，在暴露于渗透性溶质时，酶活性（半乳糖-1-磷酸尿苷酰转移酶）没有增加；因此，蛋白质合成或可能的聚合物组装被认为是添加溶质所修复的关键步骤。
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