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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Ogston A G, Wells J D

Biochem J. 1970 Aug;119(1):67-73. doi: 10.1042/bj1190067.

The measurement of osmotic pressure by means of a single bead of Sephadex (Edmond, Farquhar, Dunstone & Ogston, 1968) has been made more precise by immobilizing the bead on a fine needle. The design, calibration and use of the osmometer are described. 2. The method is particularly suitable for measuring high osmotic pressures in solutions of high-molecular-weight solutes, which must not penetrate the Sephadex to a significant extent. 3. With Sephadex G-50 the limit of precision is about 1.5cmH(2)O and the lower limit of molecular weight for a solute of compact molecular form is about 10(5). 4. The time required for each equilibration is less than 10min. 5. An impaled bead can be stored in the dry state without affecting its calibration. 6. Measurements on a sample of polyvinyl alcohol, degree of polymerization stated as 1750+/-50, gave m(n) 43000+/-3000 and A(2) (6.0+/-0.24)x10(-4).

通过将一粒葡聚糖凝胶珠固定在细针上，对渗透压的测量（埃德蒙、法夸尔、邓斯通和奥格斯顿，1968年）变得更加精确。本文描述了渗透计的设计、校准和使用方法。2. 该方法特别适用于测量高分子量溶质溶液中的高渗透压，这些溶质在很大程度上不能穿透葡聚糖凝胶。3. 使用葡聚糖G - 50时，精度极限约为1.5cmH₂O，对于紧密分子形式的溶质，分子量下限约为10⁵。4. 每次平衡所需时间少于10分钟。5. 刺穿的珠子可以在干燥状态下保存而不影响其校准。6. 对一种聚合度标明为1750±50的聚乙烯醇样品进行测量，得到数均分子量m(n)为43000±3000，第二维里系数A₂为(6.0±0.24)×10⁻⁴。

Ogston A G, Wells J D

Biochem J. 1970 Aug;119(1):67-73. doi: 10.1042/bj1190067.

The measurement of osmotic pressure by means of a single bead of Sephadex (Edmond, Farquhar, Dunstone & Ogston, 1968) has been made more precise by immobilizing the bead on a fine needle. The design, calibration and use of the osmometer are described. 2. The method is particularly suitable for measuring high osmotic pressures in solutions of high-molecular-weight solutes, which must not penetrate the Sephadex to a significant extent. 3. With Sephadex G-50 the limit of precision is about 1.5cmH(2)O and the lower limit of molecular weight for a solute of compact molecular form is about 10(5). 4. The time required for each equilibration is less than 10min. 5. An impaled bead can be stored in the dry state without affecting its calibration. 6. Measurements on a sample of polyvinyl alcohol, degree of polymerization stated as 1750+/-50, gave m(n) 43000+/-3000 and A(2) (6.0+/-0.24)x10(-4).

通过将一粒葡聚糖凝胶珠固定在细针上，对渗透压的测量（埃德蒙、法夸尔、邓斯通和奥格斯顿，1968年）变得更加精确。本文描述了渗透计的设计、校准和使用方法。2. 该方法特别适用于测量高分子量溶质溶液中的高渗透压，这些溶质在很大程度上不能穿透葡聚糖凝胶。3. 使用葡聚糖G - 50时，精度极限约为1.5cmH₂O，对于紧密分子形式的溶质，分子量下限约为10⁵。4. 每次平衡所需时间少于10分钟。5. 刺穿的珠子可以在干燥状态下保存而不影响其校准。6. 对一种聚合度标明为1750±50的聚乙烯醇样品进行测量，得到数均分子量m(n)为43000±3000，第二维里系数A₂为(6.0±0.24)×10⁻⁴。