James K, Tunstall A M, Parker A C, McCormick J N
Clin Exp Immunol. 1975 Feb;19(2):237-49.
The association of alpha2-macroglobulin (alpha2M) with the surface membranes of human peripheral blood lymphocyte preparations has been investigated by the direct immunofluorescent technique. The percentage (and total number) of lymphocytes with detectable alpha2M on their surface is significantly increased in chronic lymphocytic leukaemia. The incidence of alpha2M-positive cells in normal and pathological conditions closely parallels that observed with conjugated antiserum to the kamma and gamma light chains of human immunoglobulin. It bears no relationship however to the plasma alpha2M levels or to the age of the donor. Additional blocking studies with aggregated human IgG, soluble antigen-antibody complexes and the F(ab')2 moiety of the anti-alpha2M, and indirect immunofluorescent studies with the latter, indicate that the fluorescence observed is not due to interaction of the conjugated reagent with Fc or C3 receptors.
运用直接免疫荧光技术，对α2-巨球蛋白（α2M）与人外周血淋巴细胞制剂表面膜的关联进行了研究。慢性淋巴细胞白血病患者表面可检测到α2M的淋巴细胞百分比（及总数）显著增加。正常及病理状态下α2M阳性细胞的发生率与针对人免疫球蛋白κ和γ轻链的共轭抗血清所观察到的情况密切平行。然而，它与血浆α2M水平或供体年龄无关。用聚合人IgG、可溶性抗原-抗体复合物及抗α2M的F(ab')2片段进行的额外阻断研究，以及用后者进行的间接免疫荧光研究表明，观察到的荧光并非由于共轭试剂与Fc或C3受体相互作用所致。
