培养的人成纤维细胞对α2-巨球蛋白的合成与分泌
Synthesis and secretion of alpha2-macroglobulin by cultured human fibroblasts.
作者信息
Mosher D F, Wing D A
出版信息
J Exp Med. 1976 Feb 1;143(2):462-7. doi: 10.1084/jem.143.2.462.
Abstract
The following observations indicate that cultured human WI-38 fibroblasts synthesize and secrete alpha2-macroglobulin into serum-free medium: (a) after incubation of cultures with [35S]L-methionine, a labeled protein appeared in the medium which was precipitated by antiserum directed against alpha2-macroglobulin; (b) after incubation of cultures with [35S]L-methionine, a major band of radioactivity detected by polyacrylamide gel electrophoresis of the proteins in medium co-migrated with alpha2-macroglobulin; and (c) the amount of alpha2-macroblobulin in the medium, estimated both functionally and immunologically, increased with time in normal but not not puromycin-treated cultures.
摘要
以下观察结果表明，培养的人WI-38成纤维细胞可在无血清培养基中合成并分泌α2-巨球蛋白：（a）用[35S]L-甲硫氨酸孵育细胞培养物后，培养基中出现一种标记蛋白，该蛋白可被抗α2-巨球蛋白的抗血清沉淀；（b）用[35S]L-甲硫氨酸孵育细胞培养物后，对培养基中的蛋白质进行聚丙烯酰胺凝胶电泳检测到的一条主要放射性条带与α2-巨球蛋白共迁移；（c）在正常培养物中，通过功能和免疫方法估算，培养基中α2-巨球蛋白的量随时间增加，而在嘌呤霉素处理的培养物中则不然。
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