对受劳氏肉瘤病毒感染的小鼠胚胎成纤维细胞中环状DNA的电子显微镜研究。
Electron microscopic studies of circular DNA in mouse embryo fibroblasts infected by Rauscher leukemia virus.
作者信息
Kakefuda T, Lovinger G G, Gilden R V, Hatanaka M
出版信息
J Virol. 1977 Feb;21(2):792-5. doi: 10.1128/JVI.21.2.792-795.1977.
Abstract
Using electron microscopy, a closed circular form of DNA (4.3 mum in contour length) was detected in the nucleus of mouse embryo fibroblasts 2.5 h after infection by Rauscher murine leukemia virus. These circles were distinguishable from mitochondrial DNA by various criteria, including size, absence of secondary features, and resistance to EcoRI endonuclease.
摘要
利用电子显微镜，在劳氏鼠白血病病毒感染小鼠胚胎成纤维细胞2.5小时后，在其细胞核中检测到一种闭环形式的DNA（轮廓长度为4.3微米）。通过包括大小、无二级特征以及对EcoRI核酸内切酶的抗性等各种标准，这些环状物可与线粒体DNA区分开来。
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