乙型肝炎感染中的核心抗原与循环抗核心抗体

Core antigen and circulating anti-core antibody in hepatitis B infection.

作者信息

Howard C R, Zuckerman A J

出版信息

J Immunol Methods. 1977;14(3-4):291-301. doi: 10.1016/0022-1759(77)90140-5.

Abstract

Core antigen was obtained from the sera of persistent chronic carriers of hepatitis B virus by centrifugation and treatment with Nonidet P40 and 2-mercaptoethanol. The separated core antigen was radiolabelled and identified as a nucleoprotein structure of buoyant density 1.36 g/cm3 and possessing an isoelectric point of 4.4. This material was employed in a radioimmunoassay procedure of high sensitivity for the detection of core antibody. In a series of sera from patients with acute type B hepatitis, core antibody was demonstrated 2 to 3 weeks after the onset of jaundice during the period of surface antigenaemia. The presence of core antibody may therefore provide an accurate serological marker for the detection of active or recent virus replication in future epidemiological studies of hepatitis B infection.

摘要

核心抗原是从乙肝病毒持续慢性携带者的血清中通过离心以及用聚乙二醇辛基苯基醚（Nonidet P40）和2-巯基乙醇处理而获得的。分离出的核心抗原经放射性标记后，被鉴定为一种具有1.36克/立方厘米浮力密度且等电点为4.4的核蛋白结构。这种物质被用于一种高灵敏度放射免疫分析程序中，以检测核心抗体。在一系列急性乙型肝炎患者的血清中，黄疸出现后2至3周，在表面抗原血症期间可检测到核心抗体。因此，核心抗体的存在可能为未来乙肝感染流行病学研究中检测活跃或近期病毒复制提供一个准确的血清学标志物。