Beck E G, Holt P F, Nasrallah E T
Br J Ind Med. 1971 Apr;28(2):179-85. doi: 10.1136/oem.28.2.179.
179-185. The addition of chrysotile asbestos to monolayer cultures of peritoneal and alveolar macrophages produces an increase in membrane permeability, as measured by eosin uptake and lactic dehydrogenase activity of the supernatant fluid. The lactate synthesis is increased, however. It is suggested that the permeability of the cell membrane is increased while dust particles are being phagocytosed, which may take several hours when the particles are fibrous, but that this does not imply cell damage. Treatment of chrysotile with acid, which leaves a silica surface, results in a product that reduces lactate synthesis, implying cytotoxicity. This change is counteracted by poly(2-vinyl-pyridine 1-oxide). The polymer does not affect the properties of the native chrysotile.
179 - 185. 将温石棉添加到腹膜和肺泡巨噬细胞的单层培养物中，会使膜通透性增加，这可通过上清液中伊红摄取量和乳酸脱氢酶活性来测定。然而，乳酸合成增加。这表明在吞噬尘埃颗粒时细胞膜通透性增加，当颗粒为纤维状时这可能需要数小时，但这并不意味着细胞受损。用酸处理温石棉会留下二氧化硅表面，得到的产物会降低乳酸合成，这意味着具有细胞毒性。聚（2 - 乙烯基吡啶1 - 氧化物）可抵消这种变化。该聚合物不影响天然温石棉的性质。
