Beck E G, Holt P F, Manojlović N
Br J Ind Med. 1972 Jul;29(3):280-6. doi: 10.1136/oem.29.3.280.
280-286. The effects on macrophage cultures of glass fibre, glass powder, and chrysotile asbestos are compared. Glass fibre behaves like chrysotile in producing an increase in cell membrane permeability in cultured macrophages. This is demonstrable by the increase in lactic dehydrogenase activity in the supernatant fluid. The metabolism, measured by lactate production, is not reduced as it is when quartz is phagocytosed. Glass powder behaves like the inert dust corundum, producing little change in the number of cells stained by erythrosin B and a small increase in lactate dehydrogenase activity, both being in the range of the control. There is an increase in lactate production as a result of higher metabolism due to phagocytosis. Dusts may produce two basic effects, namely a toxic effect and change in cell membrane permeability. A non-specific effect on the cell membrane due to the slow and sometimes incomplete process of ingestion of long fibres is probably a function of the morphology, particularly the length of the fibres. A primary specific effect induced by some dusts immediately follows contact with the cell membrane.
280 - 286. 比较了玻璃纤维、玻璃粉和温石棉对巨噬细胞培养物的影响。玻璃纤维在培养的巨噬细胞中产生细胞膜通透性增加的作用与温石棉类似。这可通过上清液中乳酸脱氢酶活性的增加来证明。以乳酸产生量衡量的代谢并未像吞噬石英时那样降低。玻璃粉的作用与惰性粉尘刚玉类似，对用赤藓红B染色的细胞数量几乎没有影响，乳酸脱氢酶活性略有增加，两者均在对照范围内。由于吞噬作用导致代谢增加，乳酸产生量也会增加。粉尘可能产生两种基本效应，即毒性效应和细胞膜通透性改变。由于长纤维摄取过程缓慢且有时不完全而对细胞膜产生的非特异性效应可能是形态学的作用，特别是纤维的长度。一些粉尘与细胞膜接触后立即产生的主要特异性效应。
