Costa J, Howley P M, Legallais F, Yee C, Young N, Rabson A S
J Natl Cancer Inst. 1977 Apr;58(4):1147-9. doi: 10.1093/jnci/58.4.1147.
Primary cultures of NIH nude mouse (nu/nu) kidney cells were transformed with a human papovavirus (MMV). The transformed cell line expressed T-antigen, and MMV DNA was found to be associated with the cell DNA. When NIH nu/nu mice were inoculated with the transformed cells, they developed tumors at the injection site but failed to generate detectable levels of T-antibody. A control group of nu/+ littermates rejected the tumor inoculum but mounted an antibody response to T-antigen. It was proposed that nude mouse cells may be a suitable system to test oncogenicity of in vitro transformed cells.
用人类乳头多瘤空泡病毒（MMV）对NIH裸鼠（nu/nu）肾细胞的原代培养物进行转化。转化后的细胞系表达T抗原，并且发现MMV DNA与细胞DNA相关联。当用转化后的细胞接种NIH nu/nu小鼠时，它们在注射部位形成了肿瘤，但未能产生可检测水平的T抗体。nu/+同窝仔鼠的对照组排斥肿瘤接种物，但对T抗原产生了抗体反应。有人提出，裸鼠细胞可能是测试体外转化细胞致癌性的合适系统。
