McAllister P E, Wagner R R
J Virol. 1977 Jun;22(3):839-43. doi: 10.1128/JVI.22.3.839-843.1977.
RNA-dependent RNA polymerases were found to be associated with two salmonid rhabdoviruses: infectious hematopoietic necrosis (IHN) virus and the virus of hemorrhagic septicemia (VHS). The protein composition of these rhabdoviruses closely resembles that of rabies virus rather than that of vesicular stomatitis virus (McAllister and Wagner, 1975). The optimal temperature for in vitro transcription was found to be approximately 18 degrees C for IHN virus and approximately 15 degrees for VHS,, closely approximating optimal temperatures for growth of these viruses in salmonid cells. Unlike vesicular stomatitis virus, manganese ion (1 mM) could be used as a divalent cation substitute for magnesium ion (5 mM). The in vitro transcription products of IHN and VHS viruses hybridized completely to the homologous genome but not at all to the heterologous genome.
人们发现RNA依赖性RNA聚合酶与两种鲑鱼弹状病毒有关:传染性造血器官坏死(IHN)病毒和出血性败血症(VHS)病毒。这些弹状病毒的蛋白质组成与狂犬病病毒的蛋白质组成非常相似,而不像水疱性口炎病毒(麦卡利斯特和瓦格纳,1975年)。发现IHN病毒体外转录的最佳温度约为18摄氏度,VHS病毒约为15摄氏度,这与这些病毒在鲑鱼细胞中生长的最佳温度非常接近。与水疱性口炎病毒不同,锰离子(1 mM)可用作镁离子(5 mM)的二价阳离子替代物。IHN和VHS病毒的体外转录产物与同源基因组完全杂交,但与异源基因组完全不杂交。