Wigand R, Bachmann P, Brandner G
Arch Virol. 1981;69(1):61-9. doi: 10.1007/BF01315266.
The thermostability of isometric DNA viruses increases, if the ionic strength is diminished before heating. When unpurified virus material from cell cultures is heated under conditions (temperature, time) which lead to a reduction in infectivity by 3 to 4 log10, this loss in titer is 1 to 3 log10 less, if the ionic strength is decreased by diluting the material in dist. water (1:100) before heating. A dilution in Eagle's MEM (1:100) or a previous dialysis against water does not have the same effect. This property was found in the following members of 4 DNA virus families: adenovirus 5, herpesvirus 1, SV 40 and bovine parvovirus 1. In contrast, members of pox- (vaccinia) and picornaviruses (polio 2, coxsackie B5) were found to be less thermostable under conditions of low ionic strength. Reovirus 3 showed no difference in inactivation. The observed effect may be of practical importance for heat disinfection of viruses and for their persistence in the environment.
如果在加热前降低离子强度，等轴DNA病毒的热稳定性会增加。当来自细胞培养物的未纯化病毒材料在导致感染性降低3至4个对数10的条件（温度、时间）下加热时，如果在加热前通过在蒸馏水中将材料稀释（1:100）来降低离子强度，这种滴度损失会减少1至3个对数10。在伊格尔氏MEM中稀释（1:100）或预先对水进行透析则没有相同效果。在4个DNA病毒科的以下成员中发现了这种特性：腺病毒5型、单纯疱疹病毒1型、SV40和牛细小病毒1型。相比之下，痘病毒（痘苗病毒）和小RNA病毒（脊髓灰质炎病毒2型、柯萨奇病毒B5型）的成员在低离子强度条件下热稳定性较差。呼肠孤病毒3型在灭活方面没有差异。观察到的这种效应对于病毒的热消毒及其在环境中的持久性可能具有实际意义。
