White A, Kohler R B, Wheat L J, Sathapatayavongs B, Winn W C, Girod J C, Edelstein P H
Trans Am Clin Climatol Assoc. 1982;93:50-62.
An enzyme linked immunosorbent assay was developed to detect urinary antigen excreted by patients with Legionnaires' disease. Of 47 patients tested, antigen was detected in 39. Antigen was not detected in any of 178 urine specimens from patients with other pulmonary, bacteremic, or urinary tract infections after performance of a quick and simple confirmatory test. The assay required more time to perform than a previously described radioimmunoassay but was of equivalent sensitivity and specificity and did not require expensive equipment of contact with radioactive reagents. We conclude that enzyme linked immunosorbent assay is a rapid, sensitive, and specific means for rapidly diagnosing Legionnaires' disease which can be performed in clinical laboratories unwilling or unable to use radioisotopes.
已开发出一种酶联免疫吸附测定法来检测军团病患者尿液中排出的抗原。在检测的47例患者中，有39例检测到抗原。在对178例患有其他肺部、菌血症或尿路感染的患者的尿液标本进行快速简单的确认试验后，未检测到抗原。该测定法比先前描述的放射免疫测定法需要更多时间来完成，但灵敏度和特异性相当，且不需要昂贵设备或接触放射性试剂。我们得出结论，酶联免疫吸附测定法是一种快速、灵敏且特异的手段，可用于快速诊断军团病，能在不愿意或无法使用放射性同位素的临床实验室中进行。
