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1
Studies on the pathogenesis of fever. XVI. Purification and further chemical characterization of granulocytic pyrogen.发热发病机制的研究。十六、粒细胞热原的纯化及进一步化学特性分析
J Exp Med. 1968 Feb 1;127(2):341-57. doi: 10.1084/jem.127.2.341.
2
Studies on the pathogenesis of fever. 18. Activation of leukocytes for pyrogen production.发热的发病机制研究。18. 白细胞产热原的激活。
J Exp Med. 1970 Jan 1;131(1):179-88. doi: 10.1084/jem.131.1.179.
3
Studies on the pathogenesis of fever. XVII. The cationic control of pyrogen release from exudate granulocytes in vitro.发热发病机制的研究。十七。体外渗出性粒细胞中热原释放的阳离子控制。
J Exp Med. 1970 Jan 1;131(1):165-78. doi: 10.1084/jem.131.1.165.
4
Synthesis of endogenous pyrogen by rabbit leukocytes.兔白细胞内源性致热原的合成。
J Exp Med. 1973 May 1;137(5):1263-74. doi: 10.1084/jem.137.5.1263.
5
STUDIES ON THE PATHOGENESIS OF FEVER. XII. ELECTROLYTIC FACTORYS INFLUENCING THE RELEASE OF ENDOGENOUS PYROGEN FROM POLYMORPHONUCLEAR LEUKOCYTES.发热机制的研究。十二。影响多形核白细胞释放内源性致热原的电解因素。
J Exp Med. 1964 May 1;119(5):697-714. doi: 10.1084/jem.119.5.697.
6
STUDIES ON THE PATHOGENESIS OF FEVER. 13. THE EFFECT OF PHAGOCYTOSIS ON THE RELEASE OF ENDOGENOUS PYROGEN BY POLYMORPHONUCLEAR LEUKOCYTES.发热的发病机制研究。13. 吞噬作用对多形核白细胞释放内源性致热原的影响。
J Exp Med. 1964 May 1;119(5):715-26. doi: 10.1084/jem.119.5.715.
7
Demonstration and characterization of two distinct human leukocytic pyrogens.两种不同人类白细胞热原的证实与特性研究
J Exp Med. 1974 Jun 1;139(6):1369-81. doi: 10.1084/jem.139.6.1369.
8
Chemical characterization of a rabbit leukocytic pyrogen.兔白细胞热原的化学特性
Eur J Biochem. 1982 Oct;127(2):375-80. doi: 10.1111/j.1432-1033.1982.tb06882.x.
9
Studies on the pathogenesis of fever. VII. Preliminary chemical characterization of leucocytic pyrogen.发热的发病机制研究。VII. 白细胞致热原的初步化学特性分析。
J Exp Med. 1960 Jun 1;111(6):831-40. doi: 10.1084/jem.111.6.831.
10
Studies on the pathogenesis of fever. XIV. Further observations on the chemistry of leukocytic pyrogen.发热发病机制的研究。十四、关于白细胞致热原化学性质的进一步观察。
J Exp Med. 1966 Mar 1;123(3):433-44. doi: 10.1084/jem.123.3.433.

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1
Application of a TLR overexpression cell model in pyrogen detection.TLR 过度表达细胞模型在热原检测中的应用。
Biotechnol Bioeng. 2019 Jun;116(6):1269-1279. doi: 10.1002/bit.26936. Epub 2019 Feb 20.
2
Rabbit macrophages secrete two biochemically and immunologically distinct endogenous pyrogens.兔巨噬细胞分泌两种在生化和免疫方面不同的内源性热原。
Infect Immun. 1981 Oct;34(1):177-83. doi: 10.1128/iai.34.1.177-183.1981.
3
Studies on the active site of human leukocytic pyrogen.人白细胞热原活性部位的研究。
Inflammation. 1982 Mar;6(1):63-78. doi: 10.1007/BF00910720.
4
Further comparisons of endogenous pyrogens and leukocytic endogenous mediators.内源性致热原与白细胞内源性介质的进一步比较。
Infect Immun. 1983 Jul;41(1):6-10. doi: 10.1128/iai.41.1.6-10.1983.
5
Molecular mechanisms in endotoxin fever.内毒素发热的分子机制
Agents Actions. 1983 Aug;13(5-6):470-86. doi: 10.1007/BF02176419.
6
Comparison of endogenous pyrogens from human and rabbit leucocytes utilizing Sephadex filtration.利用葡聚糖凝胶过滤法比较人和兔白细胞内源性致热原
Yale J Biol Med. 1969 Apr;41(5):376-87.
7
Studies on the pathogenesis of fever. XX. Suppression and regeneration of pyrogen-producing capacity of exudate granulocytes.发热发病机制的研究。XX. 渗出性粒细胞产热能力的抑制与再生
J Exp Med. 1970 Jul 1;132(1):127-33. doi: 10.1084/jem.132.1.127.
8
Studies on the origin of human leukocytic pyrogen.人类白细胞致热原的起源研究。
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9
Studies on the pathogenesis of fever. XIX. Localization of pyrogen in granulocytes.发热发病机制的研究。第十九部分。热原在粒细胞中的定位。
J Exp Med. 1970 Apr 1;131(4):701-9. doi: 10.1084/jem.131.4.701.
10
Studies on the pathogenesis of fever. XVII. The cationic control of pyrogen release from exudate granulocytes in vitro.发热发病机制的研究。十七。体外渗出性粒细胞中热原释放的阳离子控制。
J Exp Med. 1970 Jan 1;131(1):165-78. doi: 10.1084/jem.131.1.165.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The properties and biologic effects of bacterial pyrogens.细菌热原的性质与生物学效应。
Medicine (Baltimore). 1950 Dec;29(4):365-400. doi: 10.1097/00005792-195012000-00003.
3
Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.碱性蛋白质和肽在聚丙烯酰胺凝胶上的圆盘电泳。
Nature. 1962 Jul 21;195:281-3. doi: 10.1038/195281a0.
4
Studies on the pathogensis of fever. IX. The production of endogenous pyrogen by polymorphonuclear leucocytes.发热的发病机制研究。IX. 多形核白细胞产生内源性致热原。
J Exp Med. 1962 Jan 1;115(1):27-36. doi: 10.1084/jem.115.1.27.
5
Studies on the pathogenesis of fever. X. The effect of certain enzyme inhibitors on the production and activity of leucocvtic pvrogen.发热发病机制的研究。十、某些酶抑制剂对白细胞致热原产生和活性的影响。
J Exp Med. 1962 Jan 1;115(1):37-47. doi: 10.1084/jem.115.1.37.
6
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
7
DISC ELECTROPHORESIS IN POLYACRYLAMIDE GELS: EXTENSION TO NEW CONDITIONS OF PH AND BUFFER.聚丙烯酰胺凝胶中的圆盘电泳：向新的pH值和缓冲液条件扩展
Ann N Y Acad Sci. 1964 Dec 28;121:373-81. doi: 10.1111/j.1749-6632.1964.tb14210.x.
8
A RAPID CHEMICAL METHOD FOR QUANTIFICATION OF LIPIDS SEPARATED BY THIN-LAYER CHROMATOGRAPHY.一种用于定量通过薄层色谱法分离的脂质的快速化学方法。
J Lipid Res. 1964 Apr;5:270-2.
9
STUDIES ON THE PATHOGENESIS OF FEVER. XII. ELECTROLYTIC FACTORYS INFLUENCING THE RELEASE OF ENDOGENOUS PYROGEN FROM POLYMORPHONUCLEAR LEUKOCYTES.发热机制的研究。十二。影响多形核白细胞释放内源性致热原的电解因素。
J Exp Med. 1964 May 1;119(5):697-714. doi: 10.1084/jem.119.5.697.
10
STUDIES ON THE PATHOGENESIS OF FEVER. 13. THE EFFECT OF PHAGOCYTOSIS ON THE RELEASE OF ENDOGENOUS PYROGEN BY POLYMORPHONUCLEAR LEUKOCYTES.发热的发病机制研究。13. 吞噬作用对多形核白细胞释放内源性致热原的影响。
J Exp Med. 1964 May 1;119(5):715-26. doi: 10.1084/jem.119.5.715.

发热发病机制的研究。十六、粒细胞热原的纯化及进一步化学特性分析

Studies on the pathogenesis of fever. XVI. Purification and further chemical characterization of granulocytic pyrogen.

作者信息

Kozak M S, Hahn H H, Lennarz W J, Wood W B

出版信息

J Exp Med. 1968 Feb 1;127(2):341-57. doi: 10.1084/jem.127.2.341.

DOI:10.1084/jem.127.2.341
PMID:5635382
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2138451/
Abstract

Small quantities of highly purified granulocytic pyrogen have been separated from contaminating proteins by disc electrophoresis in polyacrylamide gel. The biologically active material thus isolated was shown to be electrophoretically homogeneous at pH 9 and pH 3.8. Earlier work on the chemical properties of the pyrogen molecule has been extended to include: (a) estimation of its molecular weight by gel filtration; (b) demonstration of free sulfhydryl groups essential for its biological activity; and (c) evidence that it is not inactivated by exhaustive extraction with ethanolether or n-heptane.

摘要

通过聚丙烯酰胺凝胶圆盘电泳，已从污染蛋白中分离出少量高度纯化的粒细胞热原。由此分离出的生物活性物质在pH 9和pH 3.8时显示出电泳均一性。关于热原分子化学性质的早期研究已扩展至包括：(a) 通过凝胶过滤估计其分子量；(b) 证明其生物活性所必需的游离巯基；以及(c) 有证据表明，用乙醇-乙醚或正庚烷彻底萃取不会使其失活。