中国仓鼠细胞中的RNA合成。I. 间期早期和晚期核糖体RNA的差异合成速率。
RNA synthesis in Chinese hamster cells. I. Differential synthetic rate for ribosomal RNA in early and late interphase.
作者信息
Enger M D, Tobey R A, Saponara A G
出版信息
J Cell Biol. 1968 Mar;36(3):583-93. doi: 10.1083/jcb.36.3.583.
Abstract
The incorporation of methionine-methyl-(14)C into 18S ribosomal RNA of cultured Chinese hamster ovary cells in early and late interphase has been determined by zone-sedimentation analysis of phenol-extracted RNA preparations. Synchronized cell cultures were prepared for these studies by thymidine treatment and by mechanical selection of mitotic cells. The specific activity of 18S RNA labeled in late interphase was found to be 1.1-1.2 times that of 18S RNA labeled in early interphase. Upon correction for increase in RNA mass, the rate of methylation of 18S RNA in late interphase is about 1.9 times that in early interphase.
摘要
通过对苯酚提取的RNA制剂进行区带沉降分析，已测定了在早、晚间期培养的中国仓鼠卵巢细胞中蛋氨酸 - 甲基 -（14）C掺入18S核糖体RNA的情况。通过胸腺嘧啶处理和机械选择有丝分裂细胞来制备同步化细胞培养物用于这些研究。发现在晚间期标记的18S RNA的比活性是在早间期标记的18S RNA的比活性的1.1 - 1.2倍。校正RNA质量增加后，晚间期18S RNA的甲基化速率约为早间期的1.9倍。
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