Enger M D, Tobey R A
J Cell Biol. 1969 Jul;42(1):308-15. doi: 10.1083/jcb.42.1.308.
Cultures of mitotic Chinese hamster cells, prepared by mechanical selection, were pulse-labeled with methionine-methyl-(14)C or with uridine-(3)H at different stages in the life cycle. The rate of (14)C incorporation into 18S RNA was measured, as was the rate of uridine-(3)H incorporation into total RNA for both monolayer and suspension cultures. The rate of incorporation increased continuously throughout interphase in a fashion inconsistent with a gene-dosage effect upon RNA synthesis.
通过机械筛选制备的有丝分裂中国仓鼠细胞培养物，在生命周期的不同阶段用甲硫氨酸 - 甲基 -（14）C 或尿苷 -（3）H 进行脉冲标记。测量了（14）C 掺入 18S RNA 的速率，以及单层培养和悬浮培养中尿苷 -（3）H 掺入总 RNA 的速率。在整个间期，掺入速率持续增加，其方式与对 RNA 合成的基因剂量效应不一致。
