中国仓鼠细胞中的RNA合成。II. G1期RNA合成速率的增加。
RNA synthesis in Chinese hamster cells. II. Increase in rate of RNA synthesis during G1.
作者信息
Enger M D, Tobey R A
出版信息
J Cell Biol. 1969 Jul;42(1):308-15. doi: 10.1083/jcb.42.1.308.
Abstract
Cultures of mitotic Chinese hamster cells, prepared by mechanical selection, were pulse-labeled with methionine-methyl-(14)C or with uridine-(3)H at different stages in the life cycle. The rate of (14)C incorporation into 18S RNA was measured, as was the rate of uridine-(3)H incorporation into total RNA for both monolayer and suspension cultures. The rate of incorporation increased continuously throughout interphase in a fashion inconsistent with a gene-dosage effect upon RNA synthesis.
摘要
通过机械筛选制备的有丝分裂中国仓鼠细胞培养物，在生命周期的不同阶段用甲硫氨酸 - 甲基 -（14）C 或尿苷 -（3）H 进行脉冲标记。测量了（14）C 掺入 18S RNA 的速率，以及单层培养和悬浮培养中尿苷 -（3）H 掺入总 RNA 的速率。在整个间期，掺入速率持续增加，其方式与对 RNA 合成的基因剂量效应不一致。
相似文献
1
RNA synthesis in Chinese hamster cells. II. Increase in rate of RNA synthesis during G1.中国仓鼠细胞中的RNA合成。II. G1期RNA合成速率的增加。
J Cell Biol. 1969 Jul;42(1):308-15. doi: 10.1083/jcb.42.1.308.
2
RNA synthesis in Chinese hamster cells. I. Differential synthetic rate for ribosomal RNA in early and late interphase.中国仓鼠细胞中的RNA合成。I. 间期早期和晚期核糖体RNA的差异合成速率。
J Cell Biol. 1968 Mar;36(3):583-93. doi: 10.1083/jcb.36.3.583.
3
Synthesis of RNA in mammalian cells during mitosis and interphase.有丝分裂和间期哺乳动物细胞中RNA的合成。
J Cell Biol. 1967 May;33(2):265-72. doi: 10.1083/jcb.33.2.265.
4
PROTEIN SYNTHESIS AND RNA SYNTHESIS DURING MITOSIS IN ANIMAL CELLS.动物细胞有丝分裂过程中的蛋白质合成与RNA合成。
J Cell Biol. 1963 Nov;19(2):267-77. doi: 10.1083/jcb.19.2.267.
5
6
Breakdown and assembly of polyribosomes in synchronized Chinese hamster cells.同步化中国仓鼠细胞中多核糖体的分解与组装
Science. 1968 Aug 23;161(3843):791-3. doi: 10.1126/science.161.3843.791.
7
Incorporation of [3-H]uridine and [M3-14-C]methionine into Chinese-hamster cell ribonucleic acid.[3-H]尿苷和[M3-14-C]甲硫氨酸掺入中国仓鼠细胞核糖核酸的研究
Biochim Biophys Acta. 1966 Jun 22;119(3):492-500.
8
Detection by means of cell fusion of macromolecular synthesis involved in the reconstruction of the nuclear envelope in mitosis.通过细胞融合检测有丝分裂中参与核膜重建的大分子合成。
J Cell Biol. 1975 Feb;64(2):378-88. doi: 10.1083/jcb.64.2.378.
9
引用本文的文献
1
Nucleic Acid Metabolism in Germinating Onion: I. Changes in Root Tip Nucleic Acid during Germination.洋葱萌发过程中的核酸代谢：I. 根尖核酸在萌发过程中的变化。
Plant Physiol. 1971 Jul;48(1):73-81. doi: 10.1104/pp.48.1.73.
2
Studies on the proliferation of thymus cells injected into syngeneic or allogeneic irradiated mice.关于注射到同基因或异基因辐照小鼠体内的胸腺细胞增殖的研究。
Clin Exp Immunol. 1971 Feb;8(2):279-89.
3
Separation of nuclei representing different phases of the growth cycle from unsynchronized mammalian cell cultures.从非同步化的哺乳动物细胞培养物中分离代表生长周期不同阶段的细胞核。
J Cell Biol. 1970 Oct;47(1):132-9. doi: 10.1083/jcb.47.1.132.
4
The use of conventional and zonal centrifugation to study the life cycle of mammalian cells. Phospholipid and macromolecular synthesis in neoplastic mast cells.利用常规离心法和区带离心法研究哺乳动物细胞的生命周期。肿瘤性肥大细胞中的磷脂和大分子合成。
Biochem J. 1970 Sep;119(3):493-9. doi: 10.1042/bj1190493.
5
Regulation of initiation of DNA synthesis in Chinese hamster cells. I. Production of stable, reversible G1-arrested populations in suspension culture.中国仓鼠细胞中DNA合成起始的调控。I. 悬浮培养中稳定、可逆的G1期阻滞群体的产生。
J Cell Biol. 1970 Jul;46(1):151-7. doi: 10.1083/jcb.46.1.151.
6
Induced changes in the rates of uridine- 3 H uptake and incorporation during the G 1 and S periods of synchronized Chinese hamster cells.在同步化的中国仓鼠细胞的G1期和S期期间，尿苷-3H摄取率和掺入率的诱导变化。
J Cell Biol. 1972 Mar;52(3):514-25. doi: 10.1083/jcb.52.3.514.
7
Automatic monitoring of biochemical parameters in tissue culture. Studies on synchronously growing mouse myeloma cells.组织培养中生化参数的自动监测。对同步生长的小鼠骨髓瘤细胞的研究。
Biochem J. 1972 Jun;128(2):445-54. doi: 10.1042/bj1280445.
8
Ribosomal ribonucleic acid maturation in synchronous strain L cells.同步培养的L细胞中核糖体核糖核酸的成熟
In Vitro. 1973 Nov-Dec;9(3):167-75. doi: 10.1007/BF02618434.
9
Differential accumulation of virus-specific RNA during the cell cycle of adenovirus-transformed rat embyro cells.腺病毒转化的大鼠胚胎细胞在细胞周期中病毒特异性RNA的差异积累。
J Virol. 1975 Apr;15(4):806-11. doi: 10.1128/JVI.15.4.806-811.1975.
10
Temporal programming of chloroplast and cytoplasmic ribosomal RNA transcription in the synchronous cell cycle of Chlamydomonas reinhardtii.莱茵衣藻同步细胞周期中叶绿体和细胞质核糖体RNA转录的时间编程。
J Cell Biol. 1977 Feb;72(2):470-81. doi: 10.1083/jcb.72.2.470.
本文引用的文献
1
LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION.哺乳动物细胞的生命周期分析。I. 一种在生命周期内定位代谢事件的方法及其在秋水仙酰胺作用和X射线亚致死剂量照射中的应用。
Biophys J. 1963 Sep;3(5):379-97. doi: 10.1016/s0006-3495(63)86828-9.
2
Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO.一种与非典型肺炎相关的病原体在人工培养基上的生长及其被鉴定为类胸膜肺炎微生物。
Proc Natl Acad Sci U S A. 1962 Jan 15;48(1):41-9. doi: 10.1073/pnas.48.1.41.
3
Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture.哺乳动物体细胞遗传学。II. 组织培养中细胞的染色体组成。
J Exp Med. 1958 Aug 1;108(2):259-68. doi: 10.1084/jem.108.2.259.
4
Incorporation of [3-H]uridine and [M3-14-C]methionine into Chinese-hamster cell ribonucleic acid.[3-H]尿苷和[M3-14-C]甲硫氨酸掺入中国仓鼠细胞核糖核酸的研究
Biochim Biophys Acta. 1966 Jun 22;119(3):492-500.
5
RNA metabolism in the HeLa cell nucleus.海拉细胞核中的RNA代谢
J Mol Biol. 1966 May;17(1):117-30. doi: 10.1016/s0022-2836(66)80098-0.
6
Synthesis of ribosomal RNA in synchronized HeLa cells.同步化的海拉细胞中核糖体RNA的合成
Nature. 1965 Oct 30;208(5009):464-6. doi: 10.1038/208464a0.
7
Low molecular weight methylated ribonucleic acid species from Chinese hamster ovary cells. I. Isolation and characterization.来自中国仓鼠卵巢细胞的低分子量甲基化核糖核酸种类。I. 分离与特性鉴定
Biochemistry. 1969 Mar;8(3):1170-81. doi: 10.1021/bi00831a051.
8
The fractionation of nuclei and the integrity of purified nucleoli in HeLa cells.HeLa细胞中细胞核的分级分离及纯化核仁的完整性
Biochim Biophys Acta. 1968 Nov 20;169(1):188-95. doi: 10.1016/0005-2787(68)90019-1.
9
Small molecular weight monodisperse nuclear RNA.小分子单分散核RNA
J Mol Biol. 1968 Dec;38(3):289-304. doi: 10.1016/0022-2836(68)90387-2.
10
RNA synthesis in Chinese hamster cells. I. Differential synthetic rate for ribosomal RNA in early and late interphase.中国仓鼠细胞中的RNA合成。I. 间期早期和晚期核糖体RNA的差异合成速率。
J Cell Biol. 1968 Mar;36(3):583-93. doi: 10.1083/jcb.36.3.583.
Zotero 插件