MacDonald T T, Ferguson A
Gastroenterology. 1978 Mar;74(3):496-500.
The effects of chronic protozoal infection on small intestinal architecture have been examined in mice, infected with Giardia muris and Hexamita muris. Techniques used were conventional histology, quantitation of intraepithelial lymphocytes, microdissection and measurement of individual villi and crypts, and epithelial cell kinetic studies. The histology of small intestine from infected mice appeared normal apart from the intraepithelial lymphocyte numbers. Mean intraepithelial lymphocyte counts in two groups of uninfected mice were 11.6 and 13.6 per 100 epithelial cells, and in two groups of infected mice were 17.6 and 21.8. Dynamic studies showed that protozoal infection doubled the cell production per crypt per hour from mean values of 6.2, 7.3, and 8.2 in three groups of uninfected animals, to 11.8, 13.4, and 17.1 in groups of chronically infected mice. Cell production per villus was also influenced by protozoal infection, with values of 93, 99, and 101 cells per hr in groups of uninfected animals whereas in infected mice the values were 155, 162, and 180 cells per hr. Although there was no reduction in villus height in the infected animals, radioautography using [3H]thymidine confirmed that the enterocytes moved rapidly up the sides of the villi than was the case for uninfected mice.
在感染鼠贾第虫和鼠六鞭毛虫的小鼠中，研究了慢性原生动物感染对小肠结构的影响。所采用的技术包括传统组织学、上皮内淋巴细胞定量、单个绒毛和隐窝的显微切割与测量，以及上皮细胞动力学研究。除上皮内淋巴细胞数量外，感染小鼠的小肠组织学表现正常。两组未感染小鼠的上皮内淋巴细胞平均计数分别为每100个上皮细胞11.6个和13.6个，而两组感染小鼠的计数分别为17.6个和21.8个。动态研究表明，原生动物感染使每个隐窝每小时的细胞生成量增加了一倍，未感染动物三组的平均值分别为6.2、7.3和8.2，而慢性感染小鼠组则为11.8、13.4和17.1。每个绒毛的细胞生成也受到原生动物感染的影响，未感染动物组每小时的值为93、99和101个细胞，而感染小鼠组的值为每小时155、162和180个细胞。尽管感染动物的绒毛高度没有降低，但使用[3H]胸腺嘧啶核苷的放射自显影证实，与未感染小鼠相比，肠上皮细胞在绒毛侧面向上移动的速度更快。
