Richards B, Pardon J, Lilley D, Cotter R, Wooley J, Worchester D
Cell Biol Int Rep. 1977 Jan;1(1):107-16. doi: 10.1016/0309-1651(77)90017-0.
A model for the sub-structure of the core particle of the nucleosome in chromatin, based on results from neutron and x-ray scattering and other physico-chemical observations, is presented. Two disc-shaped heterotypic tetramers of histones, opposed face-to-face, form the core protein. Each tetramer is enclasped by an annulus of DNA and the two annuli are joined by a linker piece. The mode of self assembly of core particles in solution is derived as are the changes in conformation of the core particle which occur on transcription and chromosome replication.
基于中子和X射线散射结果以及其他物理化学观测结果，提出了一种染色质中核小体核心颗粒亚结构的模型。两个盘状的组蛋白异型四聚体面对面相对，形成核心蛋白。每个四聚体被一个DNA环包围，两个环通过一个连接片段相连。推导了溶液中核心颗粒的自组装模式以及转录和染色体复制时核心颗粒的构象变化。
