补体和病毒过滤对呼吸道合胞病毒中和作用的影响。
Effect of complement and viral filtration on the neutralization of respiratory syncytial virus.
作者信息
Baughman R H, Fenters J D, Marquis G S, Holper J C
出版信息
Appl Microbiol. 1968 Jul;16(7):1076-80. doi: 10.1128/am.16.7.1076-1080.1968.
The addition of 10 hemolytic units of guinea pig complement has been shown to enhance the neutralizing capacity of respiratory syncytial (RS) immune sera produced in guinea pigs and ferrets. This same immune sera, when tested without complement, had little or no neutralizing capacity. The addition of complement to RS immune horse serum did not significantly increase its neutralizing capacity. Immune horse serum effectively neutralized RS virus without complement. Other studies indicated that a 50% tissue culture infective dose of between 30 and 100 should be used in RS serum neutralization tests and that incubation should be for 90 to 105 min at room temperature. The neutralizing capacity of guinea pig immune serum was not increased by the use of filtered virus. The rate of virus neutralization, however, was increased with the addition of 10 hemolytic units of complement. The neutralizing capacity of RS immune horse serum was much greater for filtered than for unfiltered RS virus. The addition of complement increased the rate of virus neutralization but did not increase the neutralizing capacity of the horse immune serum.
已证明添加10个溶血单位的豚鼠补体可增强豚鼠和雪貂产生的呼吸道合胞体（RS）免疫血清的中和能力。同样的免疫血清，在不添加补体进行检测时，几乎没有或完全没有中和能力。向RS免疫马血清中添加补体并未显著提高其中和能力。免疫马血清在不添加补体的情况下能有效中和RS病毒。其他研究表明，在RS血清中和试验中应使用30至100之间的50%组织培养感染剂量，且在室温下孵育90至105分钟。使用过滤后的病毒并未提高豚鼠免疫血清的中和能力。然而，添加10个溶血单位的补体可提高病毒中和速率。RS免疫马血清对过滤后的RS病毒的中和能力比对未过滤的RS病毒的中和能力要强得多。添加补体提高了病毒中和速率，但并未提高马免疫血清的中和能力。
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