荧光抗体、中和抗体及补体增强中和抗体检测法用于检测呼吸道合胞病毒血清抗体的比较
Comparison of fluorescent-antibody, neutralizing-antibody, and complement-enhanced neutralizing-antibody assays for detection of serum antibody to respiratory syncytial virus.
作者信息
Kaul T N, Welliver R C, Ogra P L
出版信息
J Clin Microbiol. 1981 May;13(5):957-62. doi: 10.1128/jcm.13.5.957-962.1981.
A comparison of three assays for the detection of serum antibody to respiratory syncytial virus (RSV) was carried out on 47 serum samples obtained sequentially from infants and young children with RSV infection. Neutralizing-antibody (NA) activity was determined by a semimicromethod of plaque reduction. Complement-enhanced NA activity was determined by the addition of guinea pig complement to NA assays. RSV antibody responses in immunoglobulin G, immunoglobulin M, and immunoglobulin A classes were determined by using indirect immunofluorescence techniques for fluorescent-antibody (FAb) assay. Antibody to RSV was detectable by all three techniques as early as 4 days after the onset of illness. At all phases of illness, titers obtained by complement-enhanced NA assays were significantly greater than those obtained by NA or FAb assays (P less than 0.01). RSV-FAb titers determined in the immunoglobulin G class correlated well with those determined by complement-enhanced NA or NA assays. The data suggest that the FAb assay for detection of RSV antibody in serum is somewhat less sensitive but also less laborious and more rapid than NA assays.
对从呼吸道合胞病毒（RSV）感染的婴幼儿中连续采集的47份血清样本进行了三种检测血清抗RSV抗体方法的比较。中和抗体（NA）活性通过蚀斑减少半微量法测定。补体增强的NA活性通过在NA检测中加入豚鼠补体来测定。通过间接免疫荧光技术进行荧光抗体（FAb）检测来测定免疫球蛋白G、免疫球蛋白M和免疫球蛋白A类中的RSV抗体反应。在发病后4天，所有三种技术均能检测到抗RSV抗体。在疾病的各个阶段，补体增强NA检测获得的滴度显著高于NA或FAb检测获得的滴度（P<0.01）。免疫球蛋白G类中测定的RSV-FAb滴度与补体增强NA或NA检测测定的滴度相关性良好。数据表明，血清中检测RSV抗体的FAb检测灵敏度略低，但比NA检测更省力、更快速。
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