突触蛋白。微管蛋白和肌动蛋白的特性以及一种独特的突触后致密多肽的鉴定。
Synaptic proteins. Characterization of tubulin and actin and identification of a distinct postsynaptic density polypeptide.
作者信息
Kelly P T, Cotman C W
出版信息
J Cell Biol. 1978 Oct;79(1):173-83. doi: 10.1083/jcb.79.1.173.
Abstract
The major proteins in isolated synaptic junctions (SJs) and postsynaptic densities (PSDs) have been compared to actin, tubulin, and the major neurofilament (NF) protein by two-dimensional gel electrophoresis and tryptic peptide map analysis. These studies show: (a) tubulin is present in SJ and PSD fractions and is identical to cytoplasmic tubulin, (b) actin in these fractions is very similar to the gamma- and beta-actin found predominantly in nonmuscle cells, and (c) the major PSD protein is distinct from all other known fibrous proteins.
摘要
通过二维凝胶电泳和胰蛋白酶肽图分析，将分离出的突触连接（SJ）和突触后致密物（PSD）中的主要蛋白质与肌动蛋白、微管蛋白和主要神经丝（NF）蛋白进行了比较。这些研究表明：（a）微管蛋白存在于SJ和PSD组分中，并且与细胞质微管蛋白相同；（b）这些组分中的肌动蛋白与主要在非肌肉细胞中发现的γ-肌动蛋白和β-肌动蛋白非常相似；（c）主要的PSD蛋白与所有其他已知的纤维状蛋白不同。
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