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体外合成的鸡溶菌酶结构基因序列的克隆。

Cloning of chicken lysozyme structural gene sequences synthesized in vitro.

作者信息

Sippel A E, Land H, Lindenmaier W, Nguyen-Huu M C, Wurtz T, Timmis K N, Giesecke K, Schütz G

出版信息

Nucleic Acids Res. 1978 Sep;5(9):3275-94. doi: 10.1093/nar/5.9.3275.

DOI:10.1093/nar/5.9.3275
PMID:568256
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342248/
Abstract

Double-stranded chicken lysozyme cDNA was synthesized from an oviduct mRNA fraction enriched for lysozyme mRNA. The ds-cDNA was inserted into the BamHI site of plasmid pBR322 using chemically synthesized DNA linker molecules containing the BamHI restriction endonuclease cleavage site. After bacterial transformation, colonies carrying lysozyme DNA were identified by hybridization with highly purified lysozyme cDNA. The 555 base pairs long cloned DNA fragment of one recombinant plasmid was isolated and characterized by restriction endonuclease digestion. The DNA sequence of selected parts of the inserted DNA is as predicted from the amino acid sequence of prelysozyme. The sequence data allows the unambiguous location of the coding region within lysozyme mRNA.

摘要

双链鸡溶菌酶cDNA是从富含溶菌酶mRNA的输卵管mRNA组分中合成的。使用含有BamHI限制性内切酶切割位点的化学合成DNA连接分子，将双链cDNA插入质粒pBR322的BamHI位点。细菌转化后，通过与高度纯化的溶菌酶cDNA杂交来鉴定携带溶菌酶DNA的菌落。分离出一个重组质粒的555个碱基对长的克隆DNA片段，并通过限制性内切酶消化进行表征。插入DNA所选部分的DNA序列与前溶菌酶的氨基酸序列预测一致。序列数据允许明确确定溶菌酶mRNA内的编码区域位置。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8096/342248/407e7ca285aa/nar00470-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8096/342248/cf23f0e16825/nar00470-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8096/342248/249e957b1e35/nar00470-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8096/342248/4f562fb7e531/nar00470-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8096/342248/2276f883bfdd/nar00470-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8096/342248/407e7ca285aa/nar00470-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8096/342248/cf23f0e16825/nar00470-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8096/342248/249e957b1e35/nar00470-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8096/342248/4f562fb7e531/nar00470-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8096/342248/2276f883bfdd/nar00470-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8096/342248/407e7ca285aa/nar00470-0211-a.jpg

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引用本文的文献

1
[Gene structure and gene expression. Chicken lysozyme gene].[基因结构与基因表达。鸡溶菌酶基因]
Naturwissenschaften. 1981 Apr;68(4):170-6. doi: 10.1007/BF01047196.
2
Exons encode functional and structural units of chicken lysozyme.外显子编码鸡溶菌酶的功能和结构单元。
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5759-63. doi: 10.1073/pnas.77.10.5759.
3
Cloning and partial nucleotide sequence of human immunoglobulin mu chain cDNA from B cells and mouse-human hybridomas.来自B细胞和小鼠-人杂交瘤的人免疫球蛋白μ链cDNA的克隆及部分核苷酸序列

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ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.脱氧核糖核酸的酶促合成。十四。大肠杆菌脱氧核糖核酸聚合酶的进一步纯化及性质
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DNA organisation in the chicken lysozyme gene region.鸡溶菌酶基因区域的DNA组织
Nucleic Acids Res. 1981 Aug 11;9(15):3575-88. doi: 10.1093/nar/9.15.3575.
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Exon cloning: immunoenzymatic identification of exons of the chicken lysozyme gene.
Proc Natl Acad Sci U S A. 1982 Nov;79(22):6852-5. doi: 10.1073/pnas.79.22.6852.
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5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency.真核生物信使核糖核酸（mRNA）的5'末端序列能够高效克隆。
Nucleic Acids Res. 1981 May 25;9(10):2251-66. doi: 10.1093/nar/9.10.2251.
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The beta-globin domain in immature chicken erythrocytes: enhanced solubility is coincident with histone hyperacetylation.未成熟鸡红细胞中的β-珠蛋白结构域：溶解性增强与组蛋白高度乙酰化同时发生。
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In vitro secondary activation (memory effect) of avian vitellogenin II gene in isolated liver nuclei.鸡卵黄蛋白原II基因在离体肝细胞核中的体外二次激活（记忆效应）
Proc Natl Acad Sci U S A. 1986 Jan;83(1):43-7. doi: 10.1073/pnas.83.1.43.
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Histone acetylation in chicken erythrocytes. Rates of acetylation and evidence that histones in both active and potentially active chromatin are rapidly modified.鸡红细胞中的组蛋白乙酰化。乙酰化速率以及活性染色质和潜在活性染色质中的组蛋白均被快速修饰的证据。
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Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.来自T4噬菌体感染的大肠杆菌的一种酶对核酸的磷酸化作用。
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The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.通过聚丙烯酰胺凝胶电泳测定核糖核酸的分子量。构象变化的影响。
Biochem J. 1969 Jun;113(1):131-8. doi: 10.1042/bj1130131.
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