鸡溶菌酶基因的分离
Isolation of the lysozyme gene of chicken.
作者信息
Baldacci P, Royal A, Cami B, Perrin F, Krust A, Garapin A, Kourilsky P
出版信息
Nucleic Acids Res. 1979 Jun 25;6(8):2667-81. doi: 10.1093/nar/6.8.2667.
Abstract
The lysozyme gene has been purified by molecular cloning from two chicken gene libraries. Several recombinant phages harbouring sequences homologous to a plasmid carrying a double stranded lysozyme cDNA have been isolated. One recombinant appears to carry an entire lysozyme gene. Electron microscopic studies show that the latter is split by at least three introns. The length of the gene is about 3.9 kb, 6 times longer than lysozyme mRNA.
摘要
溶菌酶基因已通过分子克隆从两个鸡基因文库中纯化出来。已分离出几个携带与携带双链溶菌酶cDNA的质粒同源序列的重组噬菌体。其中一个重组体似乎携带了完整的溶菌酶基因。电子显微镜研究表明，后者至少被三个内含子隔开。该基因的长度约为3.9 kb，比溶菌酶mRNA长6倍。
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