Nguyen-Huu M C, Stratmann M, Groner B, Wurtz T, Land H, Giesecke K, Sippel A E, Schütz G
Proc Natl Acad Sci U S A. 1979 Jan;76(1):76-80. doi: 10.1073/pnas.76.1.76.
The organization of the chicken lysozyme gene and its neighboring sequences was examined by a comparison of the restriction map of the lysozyme structural gene with the map of the lysozyme gene in genomic DNA. Chicken DNA was cleaved with restriction endonucleases and the DNA fragments were separated by agarose gel electrophoresis. After transfer of the fragments onto nitrocellulose filters, those fragments that contain lysozyme mRNA sequences were detected by hybridization of the filters to labeled probes generated from pls-1, a recombinant plasmid carrying the lysozyme structural gene. This analysis revealed the presence of at least three intervening sequences, two of which interrupt the protein coding region and one of which is located in the 3' untranslated region. When oviduct DNA and sperm DNA were compared, no difference was observed in the size and number of restriction fragments that contain either lysozyme or ovalbumin structural gene sequences.
通过比较溶菌酶结构基因的限制性图谱与基因组DNA中溶菌酶基因的图谱，研究了鸡溶菌酶基因及其相邻序列的组织情况。用限制性内切酶切割鸡DNA，然后通过琼脂糖凝胶电泳分离DNA片段。将片段转移到硝酸纤维素滤膜上后，通过将滤膜与由携带溶菌酶结构基因的重组质粒pls-1产生的标记探针杂交，检测那些含有溶菌酶mRNA序列的片段。该分析揭示了至少存在三个间隔序列，其中两个中断蛋白质编码区，一个位于3'非翻译区。比较输卵管DNA和精子DNA时，在含有溶菌酶或卵清蛋白结构基因序列的限制性片段的大小和数量上未观察到差异。
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