Hofteig J H, Druse M J
Drug Alcohol Depend. 1978 Nov;3(6):429-34. doi: 10.1016/0376-8716(78)90015-7.
Pregnant rats were pair-fed using isocaloric control or 6.6% (v/v) ethanol liquid diets. At 18, 25, and 53 days, the in vivo incorporation of [3H] leucine and [14C] glucose into proteins and lipids of three central nervous system (CNS) myelin subfractions was examined in the offspring (control and ethanol pups) of control and ethanol-treated females. With few exceptions, the pattern of CNS myelination appeared near-normal in the ethanol pups. The ethanol pups had brain weights and total myelin protein content within the normal range. In addition, the amount of protein in light, medium, and heavy myelin was normal at 18 and 25 days. However, the ethanol pups had an excess of the chemically and morphologically immature heavy fraction at 53 days. The incorporation of [3H] leucine and [14C] glucose into myelin subfractions and separated proteins and lipids was similar in control and ethanol pups. The findings of near-normal CNS myelination in the offspring of female rats fed an ethanol liquid diet during gestation differ from our previous findings of a premature onset and slowdown of active CNS myelination in the offspring of female rats that consumed an ethanol liquid diet for one month prior to conception as well as during gestation.
将怀孕大鼠分为两组，分别用等热量对照饮食或含6.6%（v/v）乙醇的液体饮食进行配对喂养。在第18、25和53天，检测了对照组和乙醇处理组雌性大鼠后代（对照幼崽和乙醇幼崽）中，[3H]亮氨酸和[14C]葡萄糖在三种中枢神经系统（CNS）髓鞘亚组分的蛋白质和脂质中的体内掺入情况。除少数例外，乙醇幼崽的中枢神经系统髓鞘形成模式接近正常。乙醇幼崽的脑重量和总髓鞘蛋白含量在正常范围内。此外，在第18天和25天，轻、中、重髓鞘中的蛋白量正常。然而，在第53天，乙醇幼崽中化学和形态上不成熟的重组分过多。在对照幼崽和乙醇幼崽中，[3H]亮氨酸和[14C]葡萄糖掺入髓鞘亚组分以及分离的蛋白质和脂质中的情况相似。孕期食用乙醇液体饮食的雌性大鼠后代中枢神经系统髓鞘形成接近正常的这一发现，与我们之前的发现不同，之前发现受孕前一个月以及孕期食用乙醇液体饮食的雌性大鼠后代中，中枢神经系统活跃髓鞘形成提前开始且速度减慢。
