Kornfeld L, Weyzen W W
Immunology. 1968 Nov;15(5):751-64.
Peritoneal cells and spleen cells from LAF mice given three intraperitoneal immunizations of sheep red blood cells synthesized haemagglutinins after transfer to X-irradiated syngeneic recipients, either with or without a concomitant injection of antigen. Antibody formation by cells transferred with antigen resembled a secondary antibody response in intact animals. Haemagglutinins appeared rapidly and in high titre. Approximately 50 per cent of the antibodies were resistant to treatment with 2-mercaptoethanol. Antibody formation by cells transferred without further exposure to antigen differed in several respects. Haemagglutinin titres were lower. Throughout the period of observation, only 6–25 per cent of the antibodies formed were mercaptoethanol-resistant. In recipients injected with spleen cells, antibodies appeared rapidly, suggesting that mature antibody-forming cells had been transferred. However, in recipients injected with peritoneal cells from the same donors, antibodies were detected only after a delay of several days, which suggested that mature antibody-forming cells had not been transferred. Haemagglutinins were synthesized equally well after transfer of all types of peritoneal cells or of a fraction consisting almost entirely of lymphoid cells. Recipients of peritoneal cells from donors which had been given either one intraperitoneal immunization or three intravenous immunizations had no or only low haemagglutinin titres, in contrast to recipients of cells from donors given three intraperitoneal immunizations. These observations suggest that antibody formation in recipients of peritoneal cells only, i.e. without further stimulation by antigen, can be attributed to cells which are present in the peritoneal cavity of donors immunized repeatedly by the intraperitoneal route, but which are not part of the active antibody-synthesizing apparatus of the donors at the time of cell transfer. The development of these cells into antibody-forming cells can be detected only in an environment devoid of antigen and of mature antibody-forming cells. It is postulated that these cells are distinct from cells which respond on re-exposure to antigen.
给LAF小鼠进行三次腹腔注射绵羊红细胞后，其腹腔细胞和脾细胞在转移至经X射线照射的同基因受体后，无论是否同时注射抗原，均能合成血凝素。与抗原一起转移的细胞产生抗体的过程类似于完整动物中的二次抗体反应。血凝素迅速出现且滴度很高。约50%的抗体对2-巯基乙醇处理有抗性。未进一步接触抗原而转移的细胞产生抗体的情况在几个方面有所不同。血凝素滴度较低。在整个观察期间，所形成的抗体中只有6% - 25%对巯基乙醇有抗性。在注射脾细胞的受体中，抗体迅速出现，这表明已转移了成熟的抗体形成细胞。然而，在注射来自相同供体的腹腔细胞的受体中，抗体在数天后才被检测到，这表明未转移成熟的抗体形成细胞。所有类型的腹腔细胞或几乎完全由淋巴细胞组成的部分细胞转移后，血凝素的合成情况相同。与接受三次腹腔免疫的供体细胞的受体相比，接受一次腹腔免疫或三次静脉免疫的供体的腹腔细胞受体没有或只有很低的血凝素滴度。这些观察结果表明，仅接受腹腔细胞的受体（即无抗原进一步刺激）中的抗体形成可归因于反复经腹腔途径免疫的供体腹腔中存在的细胞，但在细胞转移时这些细胞并非供体活跃抗体合成装置的一部分。这些细胞发育成抗体形成细胞仅在无抗原和成熟抗体形成细胞的环境中才能被检测到。据推测，这些细胞与再次接触抗原时产生反应的细胞不同。
