Sabet T, Newlin C, Friedman H
Immunology. 1969 Apr;16(4):433-46.
Antibody formation to sheep erythrocytes, as detected both at the cellular and humoral level, was suppressed in adult mice after reticulo-endothelial cell (RES) blockade induced by intraperitoneal injection of colloidal carbon. Fewer antibody plaque-forming cells (PFC) appeared in spleens of carbon pretreated mice as compared to normal controls following intraperitoneal immunization with sheep erythrocytes. The day of peak antibody response was the same, however, for both control and carbon treated animals. There was no compensatory increase in the number of PFCs in other lymphoid organs of carbon treated animals. Similarly, carbon inoculation had no detectable effect on the number of `background' PFCs in the spleens of unstimulated mice. The time of injection of carbon in relation to time of immunization influenced the effect since injection of carbon 24–48 hours prior to RBC injection resulted in maximum immunosuppression. Injection of carbon 4–5 days before red blood cells resulted in only partial immunosuppression. Treatment with carbon 1–2 weeks prior to immunization had no detectable effect. Similarly, injection of carbon 1 or 2 days after immunization had little or no effect on the peak plaque response. The decrease in the amount of serum antibody to sheep red blood cells in carbon treated mice was not as marked as that which occurred on the individual cell level. However, most of the antibody in the sera of carbon treated animals was susceptible to 2-mercaptoethanol, even 1 or 2 weeks after immunization. On the other hand, serum antibody from control mice was mainly 2-mercaptoethanol sensitive only during the first week after immunization. Immunosuppression seemed to be related to a direct effect of carbon since the supernatant fluid obtained from carbon suspensions was not suppressive. Also, washed or dialysed carbon preparations were just as effective as the original preparation in suppressing antibody responses.
通过细胞和体液水平检测发现，成年小鼠经腹腔注射胶体碳诱导网状内皮细胞（RES）阻断后，对绵羊红细胞的抗体形成受到抑制。与用绵羊红细胞进行腹腔免疫后的正常对照相比，经碳预处理的小鼠脾脏中出现的抗体噬斑形成细胞（PFC）更少。然而，对照动物和经碳处理的动物抗体反应峰值出现的时间相同。在经碳处理的动物的其他淋巴器官中，PFC的数量没有代偿性增加。同样，碳接种对未受刺激小鼠脾脏中“背景”PFC的数量没有可检测到的影响。碳注射时间与免疫时间的关系会影响效果，因为在红细胞注射前24 - 48小时注射碳会导致最大程度的免疫抑制。在红细胞注射前4 - 5天注射碳只会导致部分免疫抑制。在免疫前1 - 2周用碳处理没有可检测到的效果。同样，在免疫后1或2天注射碳对噬斑反应峰值几乎没有影响。经碳处理的小鼠血清中针对绵羊红细胞的抗体量的减少不如在单个细胞水平上那么明显。然而，即使在免疫后1或2周，经碳处理的动物血清中的大多数抗体对2 - 巯基乙醇敏感。另一方面，对照小鼠的血清抗体仅在免疫后的第一周主要对2 - 巯基乙醇敏感。免疫抑制似乎与碳的直接作用有关，因为从碳悬浮液中获得的上清液没有抑制作用。此外，洗涤或透析后的碳制剂在抑制抗体反应方面与原始制剂一样有效。
