血浆儿茶酚胺差异测定的半自动方法。
Semi-automated method for the differential determination of plasma catecholamines.
作者信息
McCullough H
出版信息
J Clin Pathol. 1968 Nov;21(6):759-63. doi: 10.1136/jcp.21.6.759.
Abstract
A method is described for the estimation of adrenaline and noradrenaline in a 5 to 10 ml plasma sample.A batch technique of adsorption and elution from alumina is used and the final estimation is carried out using an automated fluorimetric technique based on the trihydroxyindole method. Fluorescence is stabilized first by thioglycollic acid to give noradrenaline concentrations only and then by ascorbic acid to give the total catecholamine concentration. The results of a number of catecholamine analyses are given.
摘要
本文描述了一种用于测定5至10毫升血浆样本中肾上腺素和去甲肾上腺素的方法。采用了从氧化铝上吸附和洗脱的批量技术，最终测定是使用基于三羟基吲哚法的自动荧光技术进行的。首先用巯基乙酸使荧光稳定，以仅测定去甲肾上腺素浓度，然后用抗坏血酸使荧光稳定，以测定总儿茶酚胺浓度。给出了一些儿茶酚胺分析的结果。
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本文引用的文献
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An autoanalytical method for the estimation of adrenaline and noradrenaline.
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