Keller R H, Tomasi T B
J Exp Med. 1976 May 1;143(5):1140-53. doi: 10.1084/jem.143.5.1140.
Surface-bound alpha-fetoprotein (AFP) was demonstrated by immunofluorescence on approximately 1/3 of splenic lymphocytes in chronic murine graft vs. host (GVH) reactions. Splenic lymphocytes were also shown to have a suppressed phytohemagglutinin (PHA) response compared to controls while lymph node cells from the same GVH animals revealed no surface AFP and had normal PHA responses. Splenic lymphocytes showed marked synthesis of AFP in the GVH and mixed lymphocyte culture (MLC) reactions by [14C]leucine incorporation and by radioimmunoassay in MLC supernates. Lymph node cells, however, demonstrated less synthetic activity and 14C counts in their membrane fractions were not markedly elevated.
通过免疫荧光法在慢性小鼠移植物抗宿主（GVH）反应中约1/3的脾淋巴细胞上证实了表面结合的甲胎蛋白（AFP）。与对照组相比，脾淋巴细胞对植物血凝素（PHA）的反应也受到抑制，而来自相同GVH动物的淋巴结细胞未显示表面AFP，且PHA反应正常。通过[14C]亮氨酸掺入法以及对混合淋巴细胞培养（MLC）上清液进行放射免疫测定，脾淋巴细胞在GVH和MLC反应中显示出明显的AFP合成。然而，淋巴结细胞的合成活性较低，其膜部分的14C计数没有明显升高。
