Rogers B J, Van Campen H, Ueno M, Lambert H, Bronson R, Hale R
Fertil Steril. 1979 Dec;32(6):664-70. doi: 10.1016/s0015-0282(16)44416-x.
An in vitro fertilization assay employing zona-free hamster eggs was used to analyze human spermatozoal fertilizing ability. Human spermatozoa were preincubated for 18 to 20 hours in Biggers, Whitten, and Whittingham's medium (1971) at a concentration of 1 X 10(7) sperm/ml prior to the addition of zona-free superovulated hamster eggs. Eggs were examined microscopically 2 hours later for evidence of swelling or decondensing sperm heads in the cytoplasm. A total of 6266 eggs were examined in assays for both suspected fertile and infertile donors; 50 eggs/sample were examined. The percentage fertilization was found to range from 14% to 100% in the suspected fertile group with an average of 56.3%. The sperm concentration in this fertile group ranged from 22 to 303 million/ml with an average of 114. The suspected infertile samples yielded fertilization rates of 10% or less and an average count of 50.6 million/ml. These data suggest that human spermatozoa fuse with the vitelline membrane of zona-free hamster eggs and decondense with varying efficiencies. The percentage of fertilization in this cross-species system did not show a significant correlation with sperm concentration or motility. However, suspected infertile samples always yielded 10% or less fertilization in this assay. This method may have potential value as a diagnostic tool in evaluating human spermatozoal fertilizing capacity which avoids the ethical and logistcal problems associated with fertilization of human eggs in vitro.
采用无透明带仓鼠卵的体外受精试验来分析人类精子的受精能力。在添加无透明带的超排卵仓鼠卵之前，将人类精子在比格斯、惠滕和惠廷厄姆培养基（1971年）中以1×10⁷精子/毫升的浓度预孵育18至20小时。两小时后在显微镜下检查卵子，以寻找细胞质中精子头部肿胀或解聚的证据。对疑似可育和不育供体的样本进行试验时，共检查了6266个卵子；每个样本检查50个卵子。在疑似可育组中，受精率在14%至100%之间，平均为56.3%。该可育组中的精子浓度在22至303百万/毫升之间，平均为114。疑似不育样本的受精率为10%或更低，平均计数为5060万/毫升。这些数据表明，人类精子与无透明带仓鼠卵的卵黄膜融合，并以不同效率解聚。在这个跨物种系统中，受精率与精子浓度或活力没有显著相关性。然而，在该试验中，疑似不育样本的受精率总是在10%或更低。这种方法作为评估人类精子受精能力的诊断工具可能具有潜在价值，它避免了与人类卵子体外受精相关的伦理和后勤问题。
