Kole M, Krivova A Iu, Vorob'eva L I, Bab'eva I P, Voronkova V V
Prikl Biokhim Mikrobiol. 1979 Sep-Oct;15(5):660-4.
In order to increase the yield of biotin produced by the culture Sporobolomyces pararoseus, the medium containing sucrose, asparagine, MgSO4 (NH4)2SO4, KH2PO4, vitamin complex and trace elements was optimized. With the aid of a fractional factor experiment (2(5-1)) and a complete factor experiment (2(4)), the proportion of constituents was chosen in such a way as to double biotin yield, i.e. to increase it to 55.25 micrograms/l. An enrichment of the medium with yeast autolysate, casein hydrolysate and peptone in the presence of adenine increased biotin yield to 105.7 micrograms/l and cell productivity from 6.1 to 8.0 micrograms/l dry biomass.
为了提高玫瑰色掷孢酵母培养物产生生物素的产量，对含有蔗糖、天冬酰胺、硫酸镁、硫酸铵、磷酸二氢钾、维生素复合物和微量元素的培养基进行了优化。借助部分因子试验（2(5-1)）和完全因子试验（2(4)），选择成分比例以使生物素产量翻倍，即提高到55.25微克/升。在腺嘌呤存在的情况下，用酵母自溶物、酪蛋白水解物和蛋白胨富集培养基，可使生物素产量提高到105.7微克/升，细胞生产力从6.1微克/升干生物量提高到8.0微克/升。
