Carnevali F, Morpurgo G, Tecce G
Science. 1969 Mar 21;163(3873):1331-3. doi: 10.1126/science.163.3873.1331.
The density of the cytoplasmic DNA of two strains of "petite" mutants of yeast, obtained by treatment with acriflavin and with ultraviolet light, was examined in cesium chloride density-gradient centrifugation and in all cases appeared to be less than that of the wild type. A cytoplasmic respiratory-deficient strain, treated with additional acriflavin, can show a further shift of the position of the satellite band, always in the direction of reduction of density. Also, from the p(+) x p(-) cross, p(-) strains can be recovered in which the density of the satellite DNA is different from the density of the parent p(-) strain. This finding suggests the existence of recombination in cytoplasmic DNA moleciules.
通过吖啶黄素和紫外线处理获得的两株酵母“小菌落”突变体的细胞质DNA密度，在氯化铯密度梯度离心中进行了检测，在所有情况下其密度似乎都低于野生型。用额外的吖啶黄素处理的细胞质呼吸缺陷型菌株，卫星带的位置会进一步移动，且总是朝着密度降低的方向。此外，从p(+)×p(-)杂交中，可以获得p(-)菌株，其中卫星DNA的密度与亲本p(-)菌株的密度不同。这一发现表明细胞质DNA分子中存在重组现象。
