枪乌贼巨大轴突中的铵离子电流。
Ammonium ion currents in the squid giant axon.
作者信息
Binstock L, Lecar H
出版信息
J Gen Physiol. 1969 Mar;53(3):342-61. doi: 10.1085/jgp.53.3.342.
Abstract
Voltage-clamp studies on intact and internally perfused squid giant axons demonstrate that ammonium can substitute partially for either sodium or potassium. Ammonium carries the early transient current with 0.3 times the permeability of sodium and it carries the delayed current with 0.3 times the potassium permeability. The conductance changes observed in voltage clamp show approximately the same time course in ammonium solutions as in the normal physiological solutions. These ammonium ion permeabilities account for the known effects of ammonium on nerve excitability. Experiments with the drugs tetrodotoxin (TTX) and tetraethyl ammonium chloride (TEA) demonstrate that these molecules block the early and late components of the current selectively, even when both components are carried by the same ion, ammonium.
摘要
对完整的和内部灌注的枪乌贼巨轴突进行的电压钳研究表明，铵可以部分替代钠或钾。铵携带早期瞬态电流，其通透性为钠的0.3倍，它携带延迟电流，其通透性为钾的0.3倍。在电压钳中观察到的电导变化在铵溶液中的时间进程与在正常生理溶液中大致相同。这些铵离子通透性解释了铵对神经兴奋性的已知影响。用药物河豚毒素（TTX）和氯化四乙铵（TEA）进行的实验表明，即使这两个电流成分都是由同一离子铵携带的，这些分子也能选择性地阻断电流的早期和晚期成分。
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