免疫去交感神经大鼠体内儿茶酚胺和5-羟色胺的代谢

The daily urinary excretion of normetadrenaline, metadrenaline, 4-hydroxy-3-methoxyphenylglycol, 4-hydroxy-3-methoxymandelic acid, dopamine, homovanillic acid, 5-hydroxytryptamine and 5-hydroxyindolylacetic acid has been estimated in the urine of immunosympathectomized and control rats.2. A method is given which allows separate spectrophotometric determination of 4-hydroxy-3-methoxyphenylglycol and 4-hydroxy-3-methoxymandelic acid in the same sample of rat urine. Metadrenaline and normetadrenaline were estimated by a modification of the method of Anton & Sayre (1966).3. Approximately 17% of the 4-hydroxy-3-methoxymandelic acid in rat urine is excreted in the free state, over 60% as a glucuronide conjugate and the remainder as a sulphate conjugate.4. Urinary excretion of 4-hydroxy-3-methoxyphenylglycol was approximately halved in immunosympathectomized rats (P<0.001). 4-Hydroxy-3-methoxymandelic acid output was significantly increased in treated animals (P<0.05) when expressed in terms of body weight. No significant difference was detected for any of the other compounds investigated.5. The results are discussed in the light of previous knowledge of the effect of immunosympathectomy on catecholamine metabolism.

已对免疫交感神经切除的大鼠和对照大鼠尿液中去甲变肾上腺素、变肾上腺素、4-羟基-3-甲氧基苯乙二醇、4-羟基-3-甲氧基扁桃酸、多巴胺、高香草酸、5-羟色胺和5-羟基吲哚乙酸的每日尿排泄量进行了估算。
给出了一种方法，可对大鼠尿液同一样本中的4-羟基-3-甲氧基苯乙二醇和4-羟基-3-甲氧基扁桃酸进行分光光度法单独测定。变肾上腺素和去甲变肾上腺素通过对Anton和Sayre（1966年）方法的改进进行估算。
大鼠尿液中约17%的4-羟基-3-甲氧基扁桃酸以游离状态排泄，超过60%以葡糖醛酸共轭物形式排泄，其余以硫酸盐共轭物形式排泄。
免疫交感神经切除的大鼠中4-羟基-3-甲氧基苯乙二醇的尿排泄量约减半（P<0.001）。以体重表示时，处理动物的4-羟基-3-甲氧基扁桃酸产量显著增加（P<0.05）。在所研究的任何其他化合物中均未检测到显著差异。
根据先前关于免疫交感神经切除对儿茶酚胺代谢影响的知识对结果进行了讨论。

Catecholamine and 5-hydroxyindole metabolism in immunosympathectomized rats.