Onkelinx E, Meuldermans W, Joniau M, Lontie R
Immunology. 1969 Jan;16(1):35-43.
A sensitive passive haemagglutination technique is described depending on the attachment of proteins to erythrocytes with glutaraldehyde. Various factors that play a role in the coupling of bovine serum albumin to sheep erythrocytes are examined. A standard procedure was deduced by which other proteins were coupled and tested with their specific antisera. The observed titres are comparable to those obtained with the classical bis-diazotized benzidine method. The technique is specific and practicable. It provides sensitized cells that remain stable for several weeks.
本文描述了一种灵敏的被动血凝技术，该技术依赖于用戊二醛将蛋白质附着于红细胞上。研究了在牛血清白蛋白与绵羊红细胞偶联过程中起作用的各种因素。推导了一种标准程序，通过该程序可将其他蛋白质进行偶联并用其特异性抗血清进行检测。观察到的效价与用经典的双偶氮联苯胺法获得的效价相当。该技术具有特异性且切实可行。它提供的致敏细胞可在数周内保持稳定。
