Hamilton P B, Carroll F I, Rutledge J H, Mason J E, Harris B S, Fenske C S, Wall M E
Appl Microbiol. 1969 Jan;17(1):102-5. doi: 10.1128/am.17.1.102-105.1969.
An unidentified actinomycete, RTI 246, was found to produce antimycin A(1) in high yield on a high protein cereal medium. The antibiotic compound was extracted from the cells and isolated in pure form by crystallization. It was identified by ultraviolet, infrared, nuclear magnetic resonance, and mass spectroscopy and by alkaline hydrolysis to antimycic acid and a neutral lactone. The intravenous LD(50) was 1.0 mg/kg in white mice, whereas the intraperitoneal LD(50) was 1.50 +/- 0.19 mg/kg. Animals receiving an intraperitoneal injection displayed an incoordination of the hind limbs and impaired reflexes before showing signs of respiratory distress. These findings indicated that antimycin A(1) possesses a neurotoxic property separate from its well-documented property as a respiratory poison.
一种未鉴定的放线菌RTI 246被发现能在高蛋白谷物培养基上高产抗霉素A(1)。抗生素化合物从细胞中提取出来，并通过结晶分离成纯形式。通过紫外、红外、核磁共振和质谱分析以及通过碱性水解为抗霉菌酸和中性内酯对其进行了鉴定。静脉注射半数致死量（LD(50)）在小白鼠中为1.0毫克/千克，而腹腔注射半数致死量为1.50±0.19毫克/千克。接受腹腔注射的动物在出现呼吸窘迫迹象之前，后肢表现出不协调且反射受损。这些发现表明抗霉素A(1)具有一种神经毒性特性，这与其作为呼吸毒物的广为人知的特性不同。
