Faber J, Friis T, Kirkegaard C, Siersbaek-Nielsen K
Acta Endocrinol (Copenh). 1978 Feb;87(2):313-9. doi: 10.1530/acta.0.0870313.
A simple radioimmunoassay for serum reverse triiodothyronine on small reusable Sephadex G 25 fine columns has been developed. The assay was unaffected by serum volumes of up to 100 microliter. The recovery of reverse T3 added to serum was in the mean 98.3 +/- SEM 3.2% and the coefficient of variation within and between assay determinations 5.4% and 7.5%, respectively. The detection limit was 2.2 pg reverse T3/column. Serum reverse T3 in 58 euthyroid controls was in mean 48 +/- SD 9 ng/100 ml, and was positively correlated to age (P less than 0.001). No overlap was found between the control group, and the hypo- and hyperthyroid group respectively. Three patients with T3-toxicosis had a normal serum rT3.
已开发出一种用于在小型可重复使用的葡聚糖凝胶G 25细柱上检测血清反三碘甲状腺原氨酸的简单放射免疫分析方法。该分析不受高达100微升血清体积的影响。添加到血清中的反T3回收率平均为98.3±标准误3.2%，分析测定内和测定间的变异系数分别为5.4%和7.5%。检测限为2.2皮克反T3/柱。58例甲状腺功能正常对照者的血清反T3平均为48±标准差9纳克/100毫升，且与年龄呈正相关（P<0.001）。对照组与甲状腺功能减退组和甲状腺功能亢进组之间均未发现重叠。3例T3中毒患者的血清反T3正常。
